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Invitrogen
M-CSF Monoclonal Antibody (21113)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
M-CSF Antibody (MA5-23813) in WB
Western Blot was performed using Anti-M-CSF Monoclonal Antibody (21113) (Product # MA5-23708), and a 44 kDa band corresponding to Macrophage colony-stimulating factor 1 was observed in SK-BR-3 but not in Raji which is reported to be low or negative for M-CSF expression. Whole cell extracts (60 µg lysate) of SK-BR-3 (Lane 1) and Raji (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting ... View More
Product Details
MA5-23813
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2A
Class
Monoclonal
Type
Antibody
Clone
21113
Immunogen
E. coli-derived recombinant human M-CSF
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609574
Product Specific Information
This antibody detects recombinant human (rh)M-CSF in Western blots. In sandwich ELISAs, less than 7% cross-reactivity was observed with rmM-CSF. No significant cross-reactivity or interference was observed with over 75 other cytokines tested.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: colony stimulating factor 1 (macrophage); CSF-1; H-MCSF; Lanimostim; M-MCSF; macrophage colony stimulating factor 1; Macrophage colony-stimulating factor 1; MGC31930; PG-M-CSF; Proteoglycan macrophage colony-stimulating factor
Gene Aliases: CSF-1; CSF1; MCSF
UniProt ID: (Human) P09603
Entrez Gene ID: (Human) 1435
ossification
positive regulation of cell-matrix adhesion
osteoclast proliferation
developmental process involved in reproduction
inflammatory response
transmembrane receptor protein tyrosine kinase signaling pathway
cell proliferation
positive regulation of cell proliferation
positive regulation of gene expression
regulation of macrophage derived foam cell differentiation
positive regulation of macrophage derived foam cell differentiation
positive regulation of macrophage chemotaxis
hemopoiesis
cell differentiation
macrophage differentiation
regulation of ossification
osteoclast differentiation
positive regulation of cell migration
positive regulation of cellular protein metabolic process
positive regulation of mononuclear cell proliferation
macrophage colony-stimulating factor signaling pathway
positive regulation of multicellular organism growth
monocyte activation
odontogenesis
positive regulation of odontogenesis of dentin-containing tooth
innate immune response
positive regulation of macrophage differentiation
positive regulation of monocyte differentiation
positive regulation of osteoclast differentiation
positive regulation of protein kinase activity
positive regulation of Ras protein signal transduction
homeostasis of number of cells within a tissue
branching involved in mammary gland duct morphogenesis
mammary gland fat development
mammary duct terminal end bud growth
positive regulation of macrophage colony-stimulating factor signaling pathway
positive regulation of microglial cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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