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Product Details
MA1-40079
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
ER-MP58
Immunogen
Myeloid precursor cells.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein G
Storage buffer
PBS with 0.1% BSA
Contains
0.02% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2261019
Product Specific Information
MA1-40079 detects ER-MP58, an antigen present on all mouse macrophage colony stimulating factor (M-CSF) responsive cells.
MA1-40079 has been successfully used in immunohistochemistry (frozen), ELISA, and flow cytometry applications. Typical starting dilution is 1:50.
MA1-40079 recognizes an antigen present on all mouse macrophage colony-stimulating factor (M-CSF) responsive cells in the bone marrow, including the earliest colony-forming myeloid progenitors, as well as by the majority of other myeloid precursors. The antigen is detected on a broad development range of macrophage precursor cells to the monocytic level, but also on granulocytes. Expression is rapidly lost upon maturation beyond the monocytic stage. The antigen disappears in the course of macrophage differentiation. Furthermore the antigen is clearly different from commonly used myeloid markers as Mac-1, F4/80, and Gr-1.
Target Information
M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CSF-1; H-MCSF; M-MCSF; Macrophage colony-stimulating factor 1; MGC31930; osteopetrosis; PG-M-CSF; Proteoglycan macrophage colony-stimulating factor
Gene Aliases: C87615; Csf1; Csfm; MCSF; op
UniProt ID: (Mouse) P07141
Entrez Gene ID: (Mouse) 12977
ossification
positive regulation of cell-matrix adhesion
osteoclast proliferation
immune system process
developmental process involved in reproduction
inflammatory response
transmembrane receptor protein tyrosine kinase signaling pathway
cell proliferation
positive regulation of cell proliferation
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
positive regulation of macrophage chemotaxis
macrophage differentiation
regulation of ossification
osteoclast differentiation
positive regulation of cell migration
positive regulation of cellular protein metabolic process
positive regulation of mononuclear cell proliferation
macrophage colony-stimulating factor signaling pathway
positive regulation of multicellular organism growth
odontogenesis
positive regulation of odontogenesis of dentin-containing tooth
innate immune response
positive regulation of macrophage differentiation
positive regulation of monocyte differentiation
positive regulation of osteoclast differentiation
positive regulation of protein kinase activity
positive regulation of Ras protein signal transduction
homeostasis of number of cells within a tissue
branching involved in mammary gland duct morphogenesis
mammary gland fat development
mammary duct terminal end bud growth
positive regulation of macrophage colony-stimulating factor signaling pathway
positive regulation of microglial cell migration
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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