Invitrogen

CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), NovaFluor™ Red 755, eBioscience™

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

M048T03R06-A

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.2 µg/test
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

eBioHMb1-1 (HMb1-1)

Conjugate

NovaFluor™ Red 755 NovaFluor™ Red 755 NovaFluor™ Red 755

Excitation/Emission Max

636/755 nm View spectra spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_3099197 (AB_2931038)

Product Specific Information

Description: The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110-120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f to form the VLA-1 through VLA-6 complexes, as well as with CD51. These alpha-beta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins can also interact with cellular receptors such as VCAM-1 and MadCAM-1.

Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .

Applications Reported: This eBioHMb1-1 (HMb1-1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioHMb1-1 (HMb1-1) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.

NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Excitation: 636 nm; Emission: 756 nm; Laser: 633-640 nm (Red) Laser

Target Information

ITGB1 (Integrin Subunit Beta 1, beta1 integrin subunit, GPIIa, CD29) is a 110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. ITGB1 forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CDa-f) determining the binding properties of beta1 (VLA) integrins. ITGB1 is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. Decreased expression of ITGB1 correlates with acquiring multidrug resistance of tumor cells in the presence of anti-tumor drug. ITGB1 is up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue. Further, ITGB1 also regulates tissue damage and disease symptoms related to inflammatory bowel disease. Through an ITGB1-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta. More than 8 beta subunits with numerous splice variant isoforms have been identified in mammals. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. The distribution pattern in adult tissues for integrin beta types are mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle which express the beta1D variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Beta OL; Beta oligodendroglia; CD29; Fibronectin receptor subunit beta; integrin beta 1 (fibronectin receptor beta); Integrin beta-1; Integrin beta1; integrin VLA-4 subunit beta; ITBG1D; VLA-4 subunit beta

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Gene Aliases: 4633401G24Rik; AA409975; AA960159; CD29; ENSMUSG00000051907; Fnrb; Gm9863; gpIIa; Itgb1

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UniProt ID: (Mouse) Q8BVU1, (Rat) P49134

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Entrez Gene ID: (Mouse) 16412, (Rat) 24511

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Function(s)
glycoprotein binding fibronectin binding protease binding actin binding receptor activity receptor binding integrin binding protein binding collagen binding kinase binding protein kinase binding protein domain specific binding protein complex binding peptide binding laminin binding metal ion binding protein heterodimerization activity cell adhesion molecule binding alpha-actinin binding collagen binding involved in cell-matrix adhesion integrin
Process(es)
G1/S transition of mitotic cell cycle in utero embryonic development cell fate specification tissue homeostasis cell migration involved in sprouting angiogenesis cellular calcium ion homeostasis cell adhesion leukocyte cell-cell adhesion cell-matrix adhesion calcium-independent cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway regulation of G-protein coupled receptor protein signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration visual learning regulation of collagen catabolic process positive regulation of cell-substrate adhesion positive regulation of neuron projection development cell migration formation of radial glial scaffolds positive regulation of cell migration neuron projection development negative regulation of cell projection organization cell-substrate adhesion protein transport within lipid bilayer heterotypic cell-cell adhesion negative regulation of Rho protein signal transduction positive regulation of apoptotic process stress fiber assembly positive regulation of MAPK cascade sarcomere organization negative regulation of cell differentiation negative regulation of neuron differentiation positive regulation of neuron differentiation positive regulation of endocytosis axon extension cardiac muscle tissue development dendrite morphogenesis positive regulation of peptidyl-tyrosine phosphorylation leukocyte tethering or rolling regulation of cell cycle cardiac muscle cell differentiation bicellular tight junction assembly positive regulation of establishment of protein localization to plasma membrane negative regulation of anoikis response to activity response to nutrient levels cell-cell adhesion mediated by integrin response to gonadotropin response to drug mesodermal cell differentiation maternal process involved in female pregnancy cellular response to mechanical stimulus cellular response to vitamin D cellular response to ionizing radiation response to transforming growth factor beta
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