Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), NovaFluor™ Red 755, eBioscience™
FIGURE: 1 / 2
CD29 (Integrin beta 1) Antibody (M048T03R06-A) in Flow
C57BL/6 mouse bone marrow were unstained (left) or stained with 0.2 µg of CD29 (Integrin beta 1) Monoclonal Antibody, NovaFluor Red 755 (right). All cells were co-stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and... View More
Product Details
M048T03R06-A
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
eBioHMb1-1 (HMb1-1)
Conjugate
Excitation/Emission Max
636/755 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
RRID
AB_3099197 (AB_2931038)
Product Specific Information
Description: The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110-120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f to form the VLA-1 through VLA-6 complexes, as well as with CD51. These alpha-beta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins can also interact with cellular receptors such as VCAM-1 and MadCAM-1.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
Applications Reported: This eBioHMb1-1 (HMb1-1) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioHMb1-1 (HMb1-1) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 636 nm; Emission: 756 nm; Laser: 633-640 nm (Red) Laser
Target Information
ITGB1 (Integrin Subunit Beta 1, beta1 integrin subunit, GPIIa, CD29) is a 110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. ITGB1 forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CDa-f) determining the binding properties of beta1 (VLA) integrins. ITGB1 is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. Decreased expression of ITGB1 correlates with acquiring multidrug resistance of tumor cells in the presence of anti-tumor drug. ITGB1 is up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue. Further, ITGB1 also regulates tissue damage and disease symptoms related to inflammatory bowel disease. Through an ITGB1-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta. More than 8 beta subunits with numerous splice variant isoforms have been identified in mammals. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. The distribution pattern in adult tissues for integrin beta types are mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle which express the beta1D variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Beta OL; Beta oligodendroglia; CD29; Fibronectin receptor subunit beta; integrin beta 1 (fibronectin receptor beta); Integrin beta-1; Integrin beta1; integrin VLA-4 subunit beta; ITBG1D; VLA-4 subunit beta
Gene Aliases: 4633401G24Rik; AA409975; AA960159; CD29; ENSMUSG00000051907; Fnrb; Gm9863; gpIIa; Itgb1
UniProt ID: (Mouse) Q8BVU1, (Rat) P49134
Entrez Gene ID: (Mouse) 16412, (Rat) 24511
glycoprotein binding
fibronectin binding
protease binding
actin binding
receptor activity
receptor binding
integrin binding
protein binding
collagen binding
kinase binding
protein kinase binding
protein domain specific binding
protein complex binding
peptide binding
laminin binding
metal ion binding
protein heterodimerization activity
cell adhesion molecule binding
alpha-actinin binding
collagen binding involved in cell-matrix adhesion
integrin
G1/S transition of mitotic cell cycle
in utero embryonic development
cell fate specification
tissue homeostasis
cell migration involved in sprouting angiogenesis
cellular calcium ion homeostasis
cell adhesion
leukocyte cell-cell adhesion
cell-matrix adhesion
calcium-independent cell-matrix adhesion
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
regulation of G-protein coupled receptor protein signaling pathway
positive regulation of cell proliferation
negative regulation of cell proliferation
germ cell migration
visual learning
regulation of collagen catabolic process
positive regulation of cell-substrate adhesion
positive regulation of neuron projection development
cell migration
formation of radial glial scaffolds
positive regulation of cell migration
neuron projection development
negative regulation of cell projection organization
cell-substrate adhesion
protein transport within lipid bilayer
heterotypic cell-cell adhesion
negative regulation of Rho protein signal transduction
positive regulation of apoptotic process
stress fiber assembly
positive regulation of MAPK cascade
sarcomere organization
negative regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of neuron differentiation
positive regulation of endocytosis
axon extension
cardiac muscle tissue development
dendrite morphogenesis
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte tethering or rolling
regulation of cell cycle
cardiac muscle cell differentiation
bicellular tight junction assembly
positive regulation of establishment of protein localization to plasma membrane
negative regulation of anoikis
response to activity
response to nutrient levels
cell-cell adhesion mediated by integrin
response to gonadotropin
response to drug
mesodermal cell differentiation
maternal process involved in female pregnancy
cellular response to mechanical stimulus
cellular response to vitamin D
cellular response to ionizing radiation
response to transforming growth factor beta
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support