Invitrogen

IL-13 Monoclonal Antibody (5A2), Biotin

Product Details

M130B

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG

Class

Monoclonal

Type

Antibody

Clone

5A2

Immunogen

Recombinant human IL-14

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS with 4% BSA

Contains

proprietary preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223557

Product Specific Information

M130B targets IL-13 in ELISA, and WB applications and shows reactivity with Human samples.

The M130B immunogen is recombinant human IL-14. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

M130B detects IL-13 which has a predicted molecular weight of approximately 13 kDa.

The M130B IL13 antibody (biotinylated conjugate of clone 5A2) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody P130E. Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody M130B (clone 5A2) and antibody P130E have successfully been used in combination with recombinant IL13 protein SIL13 in ELISA applications.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The CD226/CD155 interaction regulates the proinflammatory (Th1/Th17)/anti-inflammatory (Th2) balance in humans.

Journal of immunology (Baltimore, Md. : 1950)

Lozano E,Joller N,Cao Y,Kuchroo VK,Hafler DA

M130B was used in ELISA to study the role of the interaction between CD226 and CD155 in regulating the human T-cell pro-inflammatory/anti-inflammatory balance

Tue Oct 01 00:00:00 EDT 2013

Cellular immune responses in human immunodeficiency virus (HIV)-1-infected children: is immune restoration by highly active anti-retroviral therapy comparable to non-progression?

Clinical and experimental immunology

Hainaut M,Verscheure V,Ducarme M,Schandené L,Levy J,Mascart F

M130B was used in ELISA to study cellular immune responses in HIV-1-infected children

Fri Jul 01 00:00:00 EDT 2011

Fibroblasts stimulated via HLA-II molecules produce prostaglandin E₂ and regulate cytokine production from helper T cells.

Laboratory investigation; a journal of technical methods and pathology

Kato-Kogoe N,Ohyama H,Nishimura F,Meguro M,Yoshizawa S,Okada Y,Nakasho K,Yamanegi K,Yamada N,Hata M,Higashi T,Terada N,Matsushita S

M130B was used in ELISA to study the role of HLA-DQ molecules in Th-cell responses

Wed Dec 01 00:00:00 EST 2010

Can prenatal malaria exposure produce an immune tolerant phenotype? A prospective birth cohort study in Kenya.

PLoS medicine

Malhotra I,Dent A,Mungai P,Wamachi A,Ouma JH,Narum DL,Muchiri E,Tisch DJ,King CL

M130B was used in ELISA to study the effect of the prenatal malaria exposure on immune tolerance

Wed Jul 01 00:00:00 EDT 2009

Interleukin-13 mucosal production in Helicobacter pylori-related gastric diseases.

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver

Marotti B,Rocco A,De Colibus P,Compare D,de Nucci G,Staibano S,Tatangelo F,Romano M,Nardone G

M130B was used in ELISA to study the mucosal production of interleukin 13 in Helicobacter pylori-related gastric diseases

Tue Apr 01 00:00:00 EDT 2008

Modulation of the infant immune responses by the first pertussis vaccine administrations.

Vaccine

Mascart F,Hainaut M,Peltier A,Verscheure V,Levy J,Locht C

M130B was used in ELISA to study the interleukin 13 production in infants with different pertussis-tetanus-diphtheria-poliomyelitis vaccines

Thu Jan 04 00:00:00 EST 2007

Distinct Th1- and Th2-Type prenatal cytokine responses to Plasmodium falciparum erythrocyte invasion ligands.

Infection and immunity

Malhotra I,Mungai P,Muchiri E,Ouma J,Sharma S,Kazura JW,King CL

M130B was used in ELISA to characterize the prenatal immune responses against Plasmodium falciparum erythrocyte invasion ligands

Wed Jun 01 00:00:00 EDT 2005

Bordetella pertussis infection in 2-month-old infants promotes type 1 T cell responses.

Journal of immunology (Baltimore, Md. : 1950)

Mascart F,Verscheure V,Malfroot A,Hainaut M,Piérard D,Temerman S,Peltier A,Debrie AS,Levy J,Del Giudice G,Locht C

M130B was used in ELISA to investigate the effect of Bordetella pertussis infection on type 1 T cell responses

Sat Feb 01 00:00:00 EST 2003

Adaptive immune responses of patients with asthma to the attachment (G) glycoprotein of respiratory synctial virus.

The Journal of infectious diseases

Hancock GE,Scheuer CA,Sierzega R,Pryharski KS,McBride JT,Watelet LF,Tebbey PW,Smith JD

M130B was used in ELISA to study the effect of the attachment (G) glycoprotein from respiratory synctial virus on wheezing

Sat Dec 15 00:00:00 EST 2001

Bioinformatics

Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody

View more View less

Gene Aliases: IL-13; IL13; NC30; P600

View more View less

UniProt ID: (Human) P35225

View more View less

Entrez Gene ID: (Human) 3596

View more View less

Function(s)

cytokine activity interleukin-13 receptor binding protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-13 Monoclonal Antibody (5A2), Biotin

Product Details

M130B

Western Blot (WB)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!