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Invitrogen
Nanog Monoclonal Antibody (23D2-3C6), DyLight™ 650
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
Nanog Antibody (MA1-017-D650) in ICC/IF
Immunofluorescence analysis of Nanog was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Nanog Mouse Monoclonal Antibody (Product # MA1-017-D488) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight (Panel a: red). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962).... View More
Product Details
MA1-017-D650
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
23D2-3C6
Immunogen
Full-length human recombinant protein expressed in bacteria
Conjugate
Excitation/Emission Max
651/673 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with proprietary stabilizer
Contains
0.02% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536680
Product Specific Information
MA1-017-D650 has been successfully used in ICC/IF applications with human samples.
Target Information
NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
Gene Aliases: NANOG
UniProt ID: (Human) Q9H9S0
Entrez Gene ID: (Human) 79923
endodermal cell fate specification
transcription, DNA-templated
regulation of transcription, DNA-templated
cell proliferation
regulation of gene expression
stem cell population maintenance
cell differentiation
somatic stem cell population maintenance
regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of nucleic acid-templated transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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