Invitrogen

TRA-1-60 Monoclonal Antibody (TRA-1-60)

2 Published Figures

14 References

View all (11) TRA-1-60 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (7)
Published Figures (2)

TRA-1-60 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

TRA-1-60 Antibody (MA1-023) in ICC/IF

Immunofluorescent analysis of TRA-1-60 using anti-TRA-1-60 monoclonal antibody (Product # MA1-023) shows staining on the cell surface of human H9 human embryonic stem cells, indicating pluripotency. TRA-1-60 staining (green) and an overlay image of TRA-1-60 with DAPI (blue) is shown. H9 human embryonic stem cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing TRA-1-60 (Product # MA1-023) at a dilution of 1:20 over night at 4 °C, washed wit... View More

TRA-1-60 Antibody in Flow Cytometry (Flow)

TRA-1-60 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

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Product Details

MA1-023

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:20-1:100

View 11 publications 11 publications

Flow Cytometry (Flow)

0.4 µg

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Bovine, Human, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

TRA-1-60

Immunogen

Human embryonal carcinoma cell line 2102Ep.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography - MBP

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536699

Target Information

TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Establishment of the iPSC line CUIMCi005-A from a patient with Stargardt disease for retinal organoid culture.

Stem cell research

Su PY,Lee W,Zernant J,Tsang SH,Nagasaki T,Corneo B,Allikmets R

MA1-023 was used in Immunocytochemistry-immunofluorescence to establish an induced pluripotent stem cell line for retinal organoid research from a patient with mild disease features who is compound heterozygous for the frequent c.5882G>A (p.Gly1961Glu) missense variant and a c.4947delC (p.Glu1650Argfs*12) frameshift variant.

Thu Dec 01 00:00:00 EST 2022

Simultaneous high-efficiency base editing and reprogramming of patient fibroblasts.

Stem cell reports

Jalil S,Keskinen T,Maldonado R,Sokka J,Trokovic R,Otonkoski T,Wartiovaara K

MA1-023 was used in Immunocytochemistry to show that overall, this method yields tens of gene-edited hiPSC monoclonal lines with unprecedented efficiency and robustness while considerably reducing the cell culture time and thus the risk for in vitro alterations.

Tue Dec 14 00:00:00 EST 2021

Simultaneous high-efficiency base editing and reprogramming of patient fibroblasts.

Stem cell reports

Jalil S,Keskinen T,Maldonado R,Sokka J,Trokovic R,Otonkoski T,Wartiovaara K

Tue Dec 14 00:00:00 EST 2021

Generation of a human control iPS cell line (ESi080-A) from a donor with no rheumatic diseases.

Stem cell research

Castro-Viñuelas R,Sanjurjo-Rodríguez C,Piñeiro-Ramil M,Rodríguez-Fernández S,Fuentes-Boquete IM,Blanco FJ,Díaz-Prado SM

MA1-023 was used in Immunocytochemistry-immunoflourescence to report the establishment of the human iPS cell line N1-FiPS4F#7 generated from skin cells of a patient with no rheumatic diseases, thus obtaining an appropriate control iPS cell line for researchers working in the field of rheumatic diseases.

Sun Mar 01 00:00:00 EST 2020

Applying hydrodynamic pressure to efficiently generate induced pluripotent stem cells via reprogramming of centenarian skin fibroblasts.

PloS one

Vosough M,Ravaioli F,Zabulica M,Capri M,Garagnani P,Franceschi C,Piccand J,Kraus MR,Kannisto K,Gramignoli R,Strom SC

MA1-023 was used in Immunocytochemistry-immunoflourescence to show efficient generation of iPSCs from the elderly may provide a source of cells for the regeneration of tissues and organs with autologous cells as well as cellular models for the study of aging, longevity and age-related diseases.

Fri Feb 14 00:00:00 EST 2020

Functional changes of AMPA responses in human induced pluripotent stem cell-derived neural progenitors in fragile X syndrome.

Science signaling

Achuta VS,Möykkynen T,Peteri UK,Turconi G,Rivera C,Keinänen K,Castrén ML

MA1-023 was used in Immunocytochemistry-immunoflourescence to study the functional maturation of the excitatory transmission system in fragile X syndrome.

Tue Jan 16 00:00:00 EST 2018

Human pluripotent reprogramming with CRISPR activators.

Nature communications

Weltner J,Balboa D,Katayama S,Bespalov M,Krjutškov K,Jouhilahti EM,Trokovic R,Kere J,Otonkoski T

Published figure using TRA-1-60 monoclonal antibody (Product # MA1-023) in Immunofluorescence

Fri Jul 06 00:00:00 EDT 2018

Human pluripotent reprogramming with CRISPR activators.

Nature communications

Weltner J,Balboa D,Katayama S,Bespalov M,Krjutškov K,Jouhilahti EM,Trokovic R,Kere J,Otonkoski T

Published figure using TRA-1-60 monoclonal antibody (Product # MA1-023) in Immunofluorescence

Fri Jul 06 00:00:00 EDT 2018

Insulin mutations impair beta-cell development in a patient-derived iPSC model of neonatal diabetes.

eLife

Balboa D,Saarimäki-Vire J,Borshagovski D,Survila M,Lindholm P,Galli E,Eurola S,Ustinov J,Grym H,Huopio H,Partanen J,Wartiovaara K,Otonkoski T

MA1-023 was used in Immunocytochemistry to demonstrate that misfolded proinsulin impairs developing beta-cell proliferation without increasing apoptosis.

Fri Nov 09 00:00:00 EST 2018

ATPase-deficient mitochondrial inner membrane protein ATAD3A disturbs mitochondrial dynamics in dominant hereditary spastic paraplegia.

Human molecular genetics

Cooper HM,Yang Y,Ylikallio E,Khairullin R,Woldegebriel R,Lin KL,Euro L,Palin E,Wolf A,Trokovic R,Isohanni P,Kaakkola S,Auranen M,Lönnqvist T,Wanrooij S,Tyynismaa H

MA1-023 was used in Immunocytochemistry to show that mutations in ATPase family AAA-domain containing protein 3A can be dominantly inherited and underlie variable neurological phenotypes, including hereditary spastic paraplegia, with intrafamiliar variability.

Sat Apr 15 00:00:00 EDT 2017

Metabotropic glutamate receptor 5 responses dictate differentiation of neural progenitors to NMDA-responsive cells in fragile X syndrome.

Developmental neurobiology

Achuta VS,Grym H,Putkonen N,Louhivuori V,Kärkkäinen V,Koistinaho J,Roybon L,Castrén ML

MA1-023 was used in immunocytochemistry to study metabotropic glutamate receptor 5 in a murine model of fragile X syndrome

Sat Apr 01 00:00:00 EDT 2017

Amnion Epithelial Cells of Buffalo (Bubalus Bubalis) Term Placenta Expressed Embryonic Stem Cells Markers and Differentiated into Cells of Neurogenic Lineage In Vitro.

Animal biotechnology

Ghosh K,Selokar NL,Gahlawat SK,Kumar D,Kumar P,Yadav PS

MA1-023X was used in immunocytochemistry to isolate and characterize buffalo amniotic membrane-derived epithelial cells

Mon Jul 18 00:00:00 EDT 2016

Combined negative effect of donor age and time in culture on the reprogramming efficiency into induced pluripotent stem cells.

Stem cell research

Trokovic R,Weltner J,Noisa P,Raivio T,Otonkoski T

MA1-023 was used in immunocytochemistry to study the effects of donor age and passage number on the efficiency of iPSC induction

Wed Jul 01 00:00:00 EDT 2015

Generation of iPSC line HEL24.3 from human neonatal foreskin fibroblasts.

Stem cell research

Trokovic R,Weltner J,Otonkoski T

MA1-023 was used in immunocytochemistry to describe the creation of a human iPSC line HEL24.3.

Wed Jul 01 00:00:00 EDT 2015

Generation of iPSC line HEL47.2 from healthy human adult fibroblasts.

Stem cell research

Trokovic R,Weltner J,Otonkoski T

MA1-023 was used in immunocytochemistry to establish a human iPSC line

Wed Jul 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: TRA160; tra60

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TRA-1-60 Monoclonal Antibody (TRA-1-60)

TRA-1-60 Antibody (MA1-023) in ICC/IF

Product Details

MA1-023

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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