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TRA-1-81 Antibody (MA1-024-D650) in ICC/IF
Imunofluorescent analysis of TRA-1-81 (red) in human episomal iPSC cells. Cells were grown on vitronectin coated plates, fixed with 4% paraformaldehyde for 15 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with DyLight 650-conjugated TRA-1-81 monoclonal antibody (bottom right and top left panels, Product # MA1-024-D650) or DyLight 650-conjugated Mouse IgM isotype control antibody (top right panel, Product # MA1-194-D650), at a dilution of 1:250 and incubated for 1 hour at r... View More
Product Details
MA1-024-D650
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
TRA-1-81
Immunogen
human embryonal carcinoma cell line 2102Ep
Conjugate
Excitation/Emission Max
651/673 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography - MBP
Storage buffer
PBS with proprietary stabilizer
Contains
0.02% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536709
Product Specific Information
MA1-024-D650 has been successfully used in flow cytometry applications on human samples.
Target Information
TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: tra1-81; TRA180
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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