Invitrogen

CD45RO Monoclonal Antibody (UCHL1), FITC

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

11 References

View all (42) CD45RO antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Advanced Verification (1)

CD45RO Antibody in Flow Cytometry (Flow)

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CD45RO Antibody (MA1-10235) in Flow

Flow cytometry analysis (surface staining) of CD45R0 in human peripheral blood with anti-CD45R0 (UCHL1) FITC Monoclonal antibody (Product # MA1-10235).

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Product Details

MA1-10235

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

20 µL/1x10^6 cells

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

UCHL1

Immunogen

Human IL-2 dependent T cells

View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153235

Product Specific Information

This antibody recognizes an extracellular epitope of CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes.

Target Information

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A giant primary sclerosing lipogranuloma of the scrotum.

Turk patoloji dergisi

Mungan S,Karagüzel E,Turan C,Reıs A

MA1-10235 was used in immunohistochemistry to report on a case of giant primary sclerosing lipogranuloma of the scrotum

Tue Jul 08 00:00:00 EDT 2014

Axonal projections of mechanoreceptive dorsal root ganglion neurons depend on Ret.

Development (Cambridge, England)

Honma Y,Kawano M,Kohsaka S,Ogawa M

MA1-10235 was used in immunohistochemistry to study the role of Ret in the neurturin-mediated formation of precise axonal projections in the central nervous system

Thu Jul 01 00:00:00 EDT 2010

A patient with clinicopathologic features of small plaque parapsoriasis presenting later with plaque-stage mycosis fungoides: report of a case and comparative retrospective study of 27 cases of "nonprogressive" small plaque parapsoriasis.

Journal of the American Academy of Dermatology

Belousova IE,Vanecek T,Samtsov AV,Michal M,Kazakov DV

MA1-10235 was used in immunohistochemistry to report on a case of small plaque parapsoriasis with plaque-stage mycosis fungoides

Mon Sep 01 00:00:00 EDT 2008

Hyperplasia of hair follicles and other adnexal structures in cutaneous lymphoproliferative disorders: a study of 53 cases, including so-called pseudolymphomatous folliculitis and overt lymphomas.

The American journal of surgical pathology

Kazakov DV,Belousova IE,Kacerovska D,Sima R,Vanecek T,Vazmitel M,Pizinger K,Michal M

MA1-10235 was used in immunohistochemistry to study 53 cases of cutaneous lymphoproliferative disorders

Wed Oct 01 00:00:00 EDT 2008

Natural regulatory T cells: number and function are normal in the majority of patients with lupus nephritis.

Clinical and experimental immunology

Yates J,Whittington A,Mitchell P,Lechler RI,Lightstone L,Lombardi G

MA1-10235 was used in flow cytometry to examine the frequency, phenotype, and function of T regulatory cells in patients with systemic lupus erythematosus and inactive disease

Tue Jul 01 00:00:00 EDT 2008

Up-regulation of expression of the ubiquitin carboxyl-terminal hydrolase L1 gene in human airway epithelium of cigarette smokers.

Cancer research

Carolan BJ,Heguy A,Harvey BG,Leopold PL,Ferris B,Crystal RG

MA1-10235 was used in immunocytochemistry to investigate the expression of carboxyl-terminal hydrolyase L1 gene in human airway epithelium of cigarette smokers

Wed Nov 15 00:00:00 EST 2006

Primary cutaneous histiocyte and neutrophil-rich CD30+ and CD56+ anaplastic large-cell lymphoma with prominent angioinvasion and nerve involvement in the forehead and scalp of an immunocompetent woman.

Journal of cutaneous pathology

Boudova L,Kazakov DV,Jindra P,Sima R,Vanecek T,Kuntscher V,Vera V,Bouda J,Michal M

MA1-10235 was used in immunohistochemistry to report on a case of utaneous lymphoma of the head co-expressing CD56 and CD30

Tue Aug 01 00:00:00 EDT 2006

Cutaneous lymphoid hyperplasia and other lymphoid infiltrates of the breast nipple: a retrospective clinicopathologic study of fifty-six patients.

The American Journal of dermatopathology

Boudova L,Kazakov DV,Sima R,Vanecek T,Torlakovic E,Lamovec J,Kutzner H,Szepe P,Plank L,Bouda J,Hes O,Mukensnabl P,Michal M

MA1-10235 was used in immunohistochemistry to study the clinicopathological spectrum of lymphoproliferations and other lymphoid infiltration of the breast nipple

Sat Oct 01 00:00:00 EDT 2005

T-cell-rich B-cell lymphoma: a clinicopathologic study of 21 cases and comparison with 43 cases of diffuse large B-cell lymphoma.

Leukemia research

Aki H,Tuzuner N,Ongoren S,Baslar Z,Soysal T,Ferhanoglu B,Sahinler I,Aydin Y,Ulku B,Aktuglu G

MA1-10235 was used in immunohistochemistry to compare the clinocopathology of T-cell rich B-cell lymphoma and diffuse large B-cell lymphoma

Mon Mar 01 00:00:00 EST 2004

Interferon-alpha and -beta inhibit the in vitro differentiation of immunocompetent human dendritic cells from CD14(+) precursors.

Blood

McRae BL,Nagai T,Semnani RT,van Seventer JM,van Seventer GA

MA1-10235 was used in flow cytometry to test if type-I interferons influence dendritic cell differentiation and function.

Sat Jul 01 00:00:00 EDT 2000

CD3 hyporesponsiveness and in vitro apoptosis are features of T cells from both malignant and nonmalignant secondary lymphoid organs.

The Journal of clinical investigation

Agrawal S,Marquet J,Delfau-Larue MH,Copie-Bergman C,Jouault H,Reyes F,Bensussan A,Farcet JP

MA1-10235 was used in Flow Cytometry to compare tumour-infiltrating lymphocytes from B cell non-Hodgkin's lymphomas with T cells from nonmalignant secondary lymphoid organs.

Sun Nov 01 00:00:00 EST 1998

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45RO Monoclonal Antibody (UCHL1), FITC

CD45RO Antibody (MA1-10235) in Flow

Product Details

MA1-10235

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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