Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Cytokeratin 18 Monoclonal Antibody (C-04), FITC
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
Cytokeratin 18 Antibody (MA1-10326) in Flow
Flow cytometry analysis (intracellular staining) of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-Cytokeratin 18 (C-04) FITC (red) Monoclonal antibody (Product # MA1-10326). Overlay with Isotype mouse IgG1 control (PPV-06) FITC antiobody.
Product Details
MA1-10326
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mammal,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
C-04
Immunogen
Cytoskeleton preparation of epidermal carcinoma cell line A431.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11152380
Product Specific Information
This antibody reacts with cytokeratin 18 (45 kDa intracellular antigen), a member of intermediate filaments subfamily represented in epithelial tissues.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: Cell proliferation-inducing gene 46 protein; CK 18; CK-18; cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; Cytokeratin18; Endo B; K 18; K18; keratin 18, type I; keratin complex 1, acidic, gene 18; Keratin D; Keratin, type I cytoskeletal 18; Keratin-18; Keratin18; KRT 18; krt18 {ECO:0000250|UniProtKB:P05783}; type I cytoskeletal 18
Gene Aliases: CK-18; CK18; CYK18; K18; Kerd; Krt1-18; KRT18; PIG46
UniProt ID: (Human) P05783, (Rat) Q5BJY9, (Mouse) P05784
Entrez Gene ID: (Human) 3875, (Rat) 294853, (Mouse) 16668
cell cycle
anatomical structure morphogenesis
viral process
tumor necrosis factor-mediated signaling pathway
Golgi to plasma membrane CFTR protein transport
negative regulation of apoptotic process
intermediate filament cytoskeleton organization
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
cell-cell adhesion
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support