Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Nestin Monoclonal Antibody (10C2), Alexa Fluor™ 488
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
Nestin Antibody (MA1-110-A488) in ICC/IF
Immunofluorescent analysis of Nestin (green) in U2OS cells. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a Nestin mouse monoclonal antibody (Product # MA1-110-A488) at a concentration of 5 µg/mL in blocking buffer for 1 hour at room temperature. F-actin (red) was stained by Dylight 554 Phalloidin (Product #21834) and nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images ... View More
Product Details
MA1-110-A488
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
10C2
Immunogen
Human nestin protein corresponding to residues 1464-1614
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A
Storage buffer
PBS with 1.0mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
AB_2723399
Product Specific Information
MA1-110 has been successfully utilized in Western Blot, immunocytochemistry, flow cytometry, and immunofluorescence applications in human samples.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: FLJ21841; intermediate filament protein; Nestin
Gene Aliases: AA166324; C78523; ESTM46; Nbla00170; NES
UniProt ID: (Human) P48681, (Mouse) Q6P5H2
Entrez Gene ID: (Human) 10763, (Mouse) 18008
multicellular organism development
nervous system development
negative regulation of neuron apoptotic process
cell projection morphogenesis
G2/M transition of mitotic cell cycle
central nervous system development
brain development
positive regulation of intermediate filament depolymerization
embryonic camera-type eye development
negative regulation of protein binding
negative regulation of catalytic activity
stem cell proliferation
positive regulation of neural precursor cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support