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ATG9A Antibody (MA1-149) in ICC/IF
Immunofluorescent analysis of ATG9 (green) accumulation in HeLa cells in response to serum starvation (0.1% FCS, 16 hours). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an ATG9 monoclonal antibody (Product # MA1-149) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-Armenian Hamster IgG secondary antibody. Images were taken o... View More
Product Details
MA1-149
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
14F2 8B1
Immunogen
peptide sequence HPEPVPEEGSEDELPPQVHK of human ATG9A C-terminus
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536859
Product Specific Information
MA1-149 was produced in Armenian hamster and detects ATG9 in human, rat and mouse samples. MA1-149 has been successfully used in Western Blot, Immunofluorescence, and ELISA procedures. Western Blot analysis with MA1-149 shows the detection of a double band at ~85-95 kDa in human ATG9 overerexpression lysates. MA1-149 also detects additional unknown bands at ~40 and ~60 kDa. In Immunofluorescence applications, MA1-149 shows accumulation and redestribution of ATG9 in response to starvation-induced autophagosome assembly.
Target Information
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apg9; APG9 autophagy 9-like 1; APG9-like 1; Apg911; APG9A; ATG 9A; ATG9 autophagy related 9 homolog A; autophagy 9-like 1 protein; autophagy protein 9; autophagy-related 9-like 1; autophagy-related 9A; Autophagy-related protein 9A; FLJ22169; mATG9
Gene Aliases: APG9L1; Atg9; ATG9A; Atg9l1; AU019532; mATG9; MGD3208; RGD1310450
UniProt ID: (Human) Q7Z3C6, (Mouse) Q68FE2, (Rat) Q5FWU3
Entrez Gene ID: (Human) 79065, (Mouse) 245860, (Rat) 363254
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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