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Invitrogen
FAS Monoclonal Antibody (UT-1)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
FAS Antibody (MA1-19024) in WB
Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of Jurkat (Lane 1), Raji (Lane 2), MOLT-4 (Lane 3), HeLa (Lane 4), MCF7 (Lane 5) and SW-480 (Lane 6). The blot was probed with anti-FAS Monoclonal Antibody (Product # MA1-19024, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 31 kDa band corresponding to FAS receptor was observed across all the cell lines tested. HeLa, MCF7 and SW-4... View More
Product Details
MA1-19024
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
UT-1
Immunogen
T cell lymphoma cells HUT-78.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4° C, do not freeze
Shipping conditions
Wet ice
RRID
AB_1073502
Target Information
FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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