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Mouse IgG1 Isotype Control (MA1-191-D488) in ICC/IF
Imunofluorescent analysis of SSEA5 (green) and SSEA4 (red) in human episomal iPSC cells. Cells were grown on vitronectin coated plates, fixed with 4% paraformaldehyde for 15 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were co-stained with DyLight 488-conjugated SSEA5 and DyLight 650-conjugated SSEA4 monoclonal antibodies (top left panel, Product # MA1... View More
Product Details
MA1-191-D488
Applications
Tested Dilution
Western Blot (WB)
Assay-dependent
Immunohistochemistry (Paraffin) (IHC (P))
Assay-dependent
Immunohistochemistry (Frozen) (IHC (F))
Assay-dependent
Immunocytochemistry (ICC/IF)
Assay-dependent
Flow Cytometry (Flow)
Assay-dependent
Control (Ctrl)
Assay-dependent
Product Specifications
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Isotype Control
Clone
MOPC-21
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536927
Product Specific Information
This antibody can be used as a negative control in any immunoassay where a DyLight 488-conjugated primary antibody of the mouse IgG1 isotype is used. DyLight 488 has an excitation/emission of 493/518 nm.
MA1-191-D488 can be used as isotype control for Dylight 488 conjugated IgG1 mouse monoclonal antibodies in immunofluorescence and flow cytometry analysis.
Target Information
Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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