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CD45RB Antibody (MA1-19115) in IHC (P)
Immunohistochemical analysis of CD45RB was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Fo... View More
Product Details
MA1-19115
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MEM-55
Immunogen
Human thymocytes and T lymphocytes.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1072470
Product Specific Information
This antibody recognizes a siliadase-sensitive extracellular epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
Immunohistochemistry (Paraffin): No pre-treatment of tissue sections is needed.
Target Information
CD45RB is a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen
Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200
UniProt ID: (Human) P08575
Entrez Gene ID: (Human) 5788
negative regulation of T cell mediated cytotoxicity
negative regulation of cytokine-mediated signaling pathway
hematopoietic progenitor cell differentiation
immunoglobulin biosynthetic process
negative regulation of protein kinase activity
protein dephosphorylation
negative regulation of cell adhesion involved in substrate-bound cell migration
cell surface receptor signaling pathway
dephosphorylation
T cell differentiation
positive regulation of B cell proliferation
peptidyl-tyrosine dephosphorylation
B cell proliferation
positive regulation of T cell proliferation
cell cycle phase transition
positive regulation of protein kinase activity
bone marrow development
stem cell development
T cell receptor signaling pathway
B cell receptor signaling pathway
positive regulation of antigen receptor-mediated signaling pathway
release of sequestered calcium ion into cytosol
defense response to virus
regulation of cell cycle
positive regulation of hematopoietic stem cell migration
positive regulation of stem cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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