Invitrogen

CD59 Monoclonal Antibody (MEM-43)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5 Published Figures

17 References

View all (86) CD59 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD59 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

CD59 Antibody (MA1-19133) in IHC (P)

Immunohistochemistry staining of human placenta (paraffin sections) using anti-CD59 (MEM-43) Monoclonal antibody (Product # MA1-19133).

CD59 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

CD59 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

CD59 Antibody — Fig. S1. ( A ) Single-plane en face and x-z confocal images of CD59 immunofluorescence (green) in flatmounts of mouse RPE. Top (at the apical surface) and middle (through the center of the cell) slices are shown. alpha-Catenin (red) was used to label the basolateral membrane of the RPE. ( B ) Immunostaining (green) of wild-type and Abca4 -/- retinal cryosections. Left images show nonspecific signal with mouse IgG. Images on the right show CD59 immunofluorescence (green) using a mouse monoclonal antibody. White arrows: apical surface of the RPE. AF, lipofuscin autofluorescence (pink); CC, choriocapillaris; nuclear marker DAPI (blue). ( C and D ) Cytotoxicity as a function of lactate dehydrogenase release after exposure to increasing concentrations of NHS ( C ) or purified C9 in 10% C9-depleted serum ( D ). Mean +- SEM, n = 3. ** P < 0.001 relative to 50% heat-inactivated NHS. n.s., not significant. ( E ) Recovery of CD59 fluorescence at the cell surface after PI-PLC treatment in RPE exposed to 10% C9-depleted serum (gray squares) or to 60 ug/mL C9 in C9-depleted serum for 10 min (green circles). Experiments were performed in the presence of cycloheximide to prevent new protein synthesis. For each condition, values are normalized to surface CD59 levels at t = 0 after PI-PLC treatment. Mean +- SEM, n >= 240 cells per condition. * P < 0.05. ( F ) Representative immunoblot and quantification of CD59 protein levels in primary porcine RPE monolayers treated or not with A2E. Chronic: {TM}

Product Details

MA1-19133

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5-4 µg/mL

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Pig, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

MEM-43

Immunogen

Thymocytes and T lymphocytes.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073171

Product Specific Information

This antibody reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on the surface of all hematopoietic cells; it is widely present on cells in all tissues. This antibody does not compete with MEM-43/5, and has blocking activity.

A suggested positive control for this product is placenta.

Target Information

CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). CD59 is the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 also associates with C5b-8 complex and counteracts appropriate formation of cytolytic pore within the plasma membrane. Further, CD59 is a low-affinity ligand of human CD2, causes T cell costimulation, and is involved in lymphocyte signal transduction. CD59 is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. Mutations in this gene cause CD59 deficiency, a disease involving hemolytic anemia, thrombosis, and cerebral infarction. Multiple alternatively spliced transcript variants of CD59 have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

KeepEX, a simple dilution protocol for improving extracellular vesicle yields from urine.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Puhka M,Nordberg ME,Valkonen S,Rannikko A,Kallioniemi O,Siljander P,Af Hällström TM

MA119133 was used in western blot to discuss methods to increase the urinary extracellular vesicle yield

Wed Feb 15 00:00:00 EST 2017

Protective responses to sublytic complement in the retinal pigment epithelium.

Proceedings of the National Academy of Sciences of the United States of America

Tan LX,Toops KA,Lakkaraju A

MA119133 was used in immunocytochemistry and western blot to study factors that limit membrane attack complexes in models of macular degeneration

Tue Aug 02 00:00:00 EDT 2016

Protective responses to sublytic complement in the retinal pigment epithelium.

Proceedings of the National Academy of Sciences of the United States of America

Tan LX,Toops KA,Lakkaraju A

MA119133 was used in immunocytochemistry and western blot to study factors that limit membrane attack complexes in models of macular degeneration

Tue Aug 02 00:00:00 EDT 2016

Protective responses to sublytic complement in the retinal pigment epithelium.

Proceedings of the National Academy of Sciences of the United States of America

Tan LX,Toops KA,Lakkaraju A

MA119133 was used in immunocytochemistry and western blot to study factors that limit membrane attack complexes in models of macular degeneration

Tue Aug 02 00:00:00 EDT 2016

Protective responses to sublytic complement in the retinal pigment epithelium.

Proceedings of the National Academy of Sciences of the United States of America

Tan LX,Toops KA,Lakkaraju A

MA119133 was used in immunocytochemistry and western blot to study factors that limit membrane attack complexes in models of macular degeneration

Tue Aug 02 00:00:00 EDT 2016

CD59 signaling and membrane pores drive Syk-dependent erythrocyte necroptosis.

Cell death & disease

LaRocca TJ,Stivison EA,Mal-Sarkar T,Hooven TA,Hod EA,Spitalnik SL,Ratner AJ

MA119133 was used in immunoprecipitation to study mechanisms of programed cell death of red blood cells

Thu May 28 00:00:00 EDT 2015

Identification of a cross-reacting, monoclonal anti-human CD233 antibody for identification and sorting of rhesus macaque erythrocytes.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Byrnes C,Terry Lee Y,Donahue RE,Miller JL

MA1-19133 was used in flow cytometry to use cross reactive antibodies to identify rhesus macaque erythrocytes.

Wed Feb 01 00:00:00 EST 2012

Human placenta and chorion: potential additional sources of hematopoietic stem cells for transplantation.

Transfusion

Bárcena A,Muench MO,Kapidzic M,Gormley M,Goldfien GA,Fisher SJ

MA1-19133 was used in immunocytochemistry to discuss the use of umbilical cord blood as a source of transplantable hematopoietic stem cells.

Tue Nov 01 00:00:00 EDT 2011

Guidelines for the diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria and related disorders by flow cytometry.

Cytometry. Part B, Clinical cytometry

Borowitz MJ,Craig FE,Digiuseppe JA,Illingworth AJ,Rosse W,Sutherland DR,Wittwer CT,Richards SJ

MA1-19133 was used in flow cytometry to discuss ways to standardize flow cytometry methods to diagnose paroxysmal nocturnal hemoglobinuria

Thu Jul 01 00:00:00 EDT 2010

Copy number of FCGR3B, which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake.

The Journal of experimental medicine

Willcocks LC,Lyons PA,Clatworthy MR,Robinson JI,Yang W,Newland SA,Plagnol V,McGovern NN,Condliffe AM,Chilvers ER,Adu D,Jolly EC,Watts R,Lau YL,Morgan AW,Nash G,Smith KG

MA1-19133 was used in flow cytometry to examine how FCGR3B copy number affects immune responses

Mon Jul 07 00:00:00 EDT 2008

Thrice-weekly low-dose rituximab decreases CD20 loss via shaving and promotes enhanced targeting in chronic lymphocytic leukemia.

Journal of immunology (Baltimore, Md. : 1950)

Williams ME,Densmore JJ,Pawluczkowycz AW,Beum PV,Kennedy AD,Lindorfer MA,Hamil SH,Eggleton JC,Taylor RP

MA1-19133 was used in flow cytometry to examine loss of CD20 in chronic lymphocytic leukemia

Wed Nov 15 00:00:00 EST 2006

Signature of B-CLL with different prognosis by Shrunken centroids of surface antigen expression profiling.

Journal of cellular physiology

Zucchetto A,Sonego P,Degan M,Bomben R,Dal Bo M,Russo S,Attadia V,Rupolo M,Buccisano F,Del Principe MI,Del Poeta G,Pucillo C,Colombatti A,Campanini R,Gattei V

MA1-19133 was used in flow cytometry to use flow cytometry to characterize 123 cases of B-cell chronic lymphocytic leukemia

Fri Jul 01 00:00:00 EDT 2005

Searching for common stem cells of the hepatic and hematopoietic systems in the human fetal liver: CD34+ cytokeratin 7/8+ cells express markers for stellate cells.

Journal of hepatology

Suskind DL,Muench MO

MA1-19133 was used in flow cytometry to test if human fetal liver cells expressing CD34 and CK7/8 represent a common stem cell for both the hematopoietic and hepatic systems

Sun Feb 01 00:00:00 EST 2004

Identification of unsuspected PNH-type cells in flow cytometric immunophenotypic analysis of peripheral blood and bone marrow.

American journal of clinical pathology

Thomason RW,Papiez J,Lee RV,Szczarkowski W

MA1-19133 was used in flow cytometry to use flow cytometry to characterize cells from patients with paroxysmal nocturnal hemoglobinuria

Thu Jul 01 00:00:00 EDT 2004

Systemic therapy with cyclophosphamide and anti-CD20 antibody (rituximab) in relapsed primary cutaneous B-cell lymphoma: a report of 7 cases.

Journal of the American Academy of Dermatology

Fierro MT,Savoia P,Quaglino P,Novelli M,Barberis M,Bernengo MG

MA1-19133 was used in immunohistochemistry to discuss the use of Rituximab.

Fri Aug 01 00:00:00 EDT 2003

The association between glycosylphosphatidylinositol-anchored proteins and heterotrimeric G protein alpha subunits in lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America

Solomon KR,Rudd CE,Finberg RW

MA1-19133 was used in immunoprecipitation to report that GPI-anchored proteins associate with G proteins.

Tue Jun 11 00:00:00 EDT 1996

Complement regulatory molecules on human myelin and glial cells: differential expression affects the deposition of activated complement proteins.

Journal of neurochemistry

Koski CL,Estep AE,Sawant-Mane S,Shin ML,Highbarger L,Hansch GM

MA1-19133 was used in western blot to study CD55 and CD59 in Schwann cells.

Mon Jan 01 00:00:00 EST 1996

Phenotypic and functional analysis of lymphocytes in paroxysmal nocturnal hemoglobinuria.

American journal of hematology

Tseng JE,Hall SE,Howard TA,Ware RE

MA1-19133 was used in immunoprecipitation to determine the lymphoid lineage involvement in paroxysmal nocturnal hemoglobinuria.

Fri Dec 01 00:00:00 EST 1995

The CD59 antigen is a structural homologue of murine Ly-6 antigens but lacks interferon inducibility.

European journal of immunology

Philbrick WM,Palfree RG,Maher SE,Bridgett MM,Sirlin S,Bothwell AL

MA1-19133 was used in flow cytometry to study expression of CD59.

Mon Jan 01 00:00:00 EST 1990

Bioinformatics

Protein Aliases: 1F5 antigen; 20 kDa homologous restriction factor; CD59; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); CD59 glycoprotein; CD59 molecule, complement regulatory protein; HRF-20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MAC-inhibitory protein; MAC-IP; MACIF; MEM43 antigen; membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane inhibitor of reactive lysis; MIRL; Protectin; surface anitgen recognized by monoclonal antibody 16.3A5; T cell-activating protein

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Gene Aliases: 16.3A5; 1F5; CD59; EJ16; EJ30; EL32; G344; HRF-20; HRF20; MAC-IP; MACIF; MEM43; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20

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UniProt ID: (Human) P13987

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Entrez Gene ID: (Human) 966

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Function(s)

complement binding protein binding

Process(es)

cell activation negative regulation of activation of membrane attack complex ER to Golgi vesicle-mediated transport cell surface receptor signaling pathway blood coagulation regulation of complement activation positive regulation of T cell proliferation negative regulation of apoptotic process COPII vesicle coating

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CD59 Monoclonal Antibody (MEM-43)

CD59 Antibody (MA1-19133) in IHC (P)

Product Details

MA1-19133

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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