Invitrogen

alpha Tubulin Monoclonal Antibody (TU-01)

2 Published Figures

34 References

View all (82) alpha Tubulin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (16)
Published Figures (2)

alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

alpha Tubulin Antibody (MA1-19162) in ICC/IF

Immunocytochemistry staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01; green) Monoclonal antibody (Product # MA1-19162) and anti-Vimentin (VI-01; red). Nucleus is stained with DAPI (blue).

alpha Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Tubulin Antibody in Western Blot (WB)

alpha Tubulin Antibody in Flow Cytometry (Flow)

alpha Tubulin Antibody in Immunoprecipitation (IP)

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Product Details

MA1-19162

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

View 4 publications 4 publications

Flow Cytometry (Flow)

1-4 µg/mL

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human, Many, Mouse, Pig

Published species

Bovine, Human, Mouse, Non-human primate, Pig, Protozoa, Rat, Xenopus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TU-01

Immunogen

Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1070259

Product Specific Information

This antibody recognizes a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.

HPB-ALL human peripheral blood leukemia cell line is the suggested positive control (incubate for 90 mins).

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

Western Blot: Reducing conditions.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Novel Fluorescent Benzimidazole-Hydrazone-Loaded Micellar Carriers for Controlled Release: Impact on Cell Toxicity, Nuclear and Microtubule Alterations in Breast Cancer Cells.

Pharmaceutics

Bryaskova R,Georgiev N,Philipova N,Bakov V,Anichina K,Argirova M,Apostolova S,Georgieva I,Tzoneva R

MA1-19162 was used in Immunocytochemistry-immunofluorescence to develop fluorescent micellar carriers with controlled release of a novel anticancer drug, leading to enhanced antiproliferative and cytotoxic effects on MDA-MB-231 cancer cells while having relatively weak effects on non-cancerous cells MCF-10A.

Fri Jun 16 00:00:00 EDT 2023

Structural basis of tankyrase activation by polymerization.

Nature

Pillay N,Mariotti L,Zaleska M,Inian O,Jessop M,Hibbs S,Desfosses A,Hopkins PCR,Templeton CM,Beuron F,Morris EP,Guettler S

MA1-19162 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to reveal a novel SAM domain polymerization mode, illustrate how supramolecular assembly controls catalytic and non-catalytic functions and provide important structural insights into the regulation of a non-DNA-dependent poly-ADP-ribosyltransferase.

Thu Dec 01 00:00:00 EST 2022

The Mediator Subunit, Med23 Is Required for Embryonic Survival and Regulation of Canonical WNT Signaling During Cranial Ganglia Development.

Frontiers in physiology

Dash S,Bhatt S,Sandell LL,Seidel CW,Ahn Y,Krumlauf RE,Trainor PA

MA1-19162 was used in Western Blotting to reveal a surprisingly tissue specific role for the ubiquitously expressed mediator complex protein Med23 in placode differentiation during cranial ganglia development.

Tue Nov 17 00:00:00 EST 2020

The Mediator Subunit, Med23 Is Required for Embryonic Survival and Regulation of Canonical WNT Signaling During Cranial Ganglia Development.

Frontiers in physiology

Dash S,Bhatt S,Sandell LL,Seidel CW,Ahn Y,Krumlauf RE,Trainor PA

Tue Nov 17 00:00:00 EST 2020

Lead optimization of selective tubulin inhibitors as anti-trypanosomal agents.

Bioorganic & medicinal chemistry

Zhao A,Li Y,Orahoske CM,Schnur B,Sabbagh A,Zhang W,Li B,Su B

MA1-19162 was used in Western Blotting to improve the ligand efficiency and reduce overall hydrophobicity of the compounds, by lead optimisation.

Mon Apr 15 00:00:00 EDT 2019

Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesis.

BMC veterinary research

Bernardini C,Bertocchi M,Zannoni A,Salaroli R,Tubon I,Dothel G,Fernandez M,Bacci ML,Calzà L,Forni M

MA1-19162 was used in Western Blotting to study the future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine.

Sat Apr 27 00:00:00 EDT 2019

SETD2 Haploinsufficiency for Microtubule Methylation Is an Early Driver of Genomic Instability in Renal Cell Carcinoma.

Cancer research

Chiang YC,Park IY,Terzo EA,Tripathi DN,Mason FM,Fahey CC,Karki M,Shuster CB,Sohn BH,Chowdhury P,Powell RT,Ohi R,Tsai YS,de Cubas AA,Khan A,Davis IJ,Strahl BD,Parker JS,Dere R,Walker CL,Rathmell WK

MA1-19162 was used in Western Blotting to study the effect of the SETD2 tumor suppressor and suggest an involvement in promoting oncogenesis in renal cell carcinoma.

Fri Jun 15 00:00:00 EDT 2018

Reduced-HMGB1 suppresses poly(I:C)-induced inflammation in keratinocytes.

Journal of dermatological science

Mori H,Murakami M,Tsuda T,Kameda K,Utsunomiya R,Masuda K,Shiraishi K,Dai X,Tohyama M,Nakaoka H,Sayama K

MA1-19162 was used in Western Blotting to evaluate the effect of reduced-HMGB1 on polyinosine-polycytidylic acid-induced inflammation in normal human keratinocytes.

Tue May 01 00:00:00 EDT 2018

Understanding the importance of conservative hypothetical protein LdBPK_070020 in Leishmania donovani and its role in subsistence of the parasite.

Archives of biochemistry and biophysics

Bhardwaj R,Kumar R,Singh SK,Selvaraj C,Dubey VK

MA1-19162 was used in western blot to study the role in subsistence of the parasite and its importance of conservative hypothetical protein LdBPK_070020 in Leishmania donovani

Fri Apr 15 00:00:00 EDT 2016

Epidermal Snail expression drives skin cancer initiation and progression through enhanced cytoprotection, epidermal stem/progenitor cell expansion and enhanced metastatic potential.

Cell death and differentiation

De Craene B,Denecker G,Vermassen P,Taminau J,Mauch C,Derore A,Jonkers J,Fuchs E,Berx G

Published figure using alpha Tubulin monoclonal antibody (Product # MA1-19162) in Immunofluorescence

Sat Feb 01 00:00:00 EST 2014

Epidermal Snail expression drives skin cancer initiation and progression through enhanced cytoprotection, epidermal stem/progenitor cell expansion and enhanced metastatic potential.

Cell death and differentiation

De Craene B,Denecker G,Vermassen P,Taminau J,Mauch C,Derore A,Jonkers J,Fuchs E,Berx G

Published figure using alpha Tubulin monoclonal antibody (Product # MA1-19162) in Immunofluorescence

Sat Feb 01 00:00:00 EST 2014

Methoxychlor and triclosan stimulates ovarian cancer growth by regulating cell cycle- and apoptosis-related genes via an estrogen receptor-dependent pathway.

Environmental toxicology and pharmacology

Kim JY,Yi BR,Go RE,Hwang KA,Nam KH,Choi KC

MA1-19162 was used in western blot to study the role of ER-dependent modulation of cell cycle and apoptosis genes in the mechanism by which methoxychlor and triclosan promote the growth of ovarian cancer

Thu May 01 00:00:00 EDT 2014

Sera from early-onset, severely preeclamptic women directly modulate HLA-E expression in the EA.hy296 endothelial cell line.

Journal of reproductive immunology

Bueno-Sánchez JC,Peña-Alzate S,Peña RB,Agudelo-Jaramillo B,Cadavid-Jaramillo AP,Chaouat G,Maldonado-Estrada JG

MA1-19162 was used in western blot to study the modulation of endothelial cell line expression of HLA-E by sera obtained from women with severe early onset preeclampsia

Wed Oct 01 00:00:00 EDT 2014

Proteinase-activated receptor 2 expression in the intestinal tract of the horse.

Research in veterinary science

Zannoni A,Bombardi C,Dondi F,Morini M,Forni M,Chiocchetti R,Spadari A,Romagnoli N

MA1-19162 was used in western blot to study the equine intestinal expression of PAR2

Sun Jun 01 00:00:00 EDT 2014

A possible involvement of p62/sequestosome-1 in the process of biliary epithelial autophagy and senescence in primary biliary cirrhosis.

Liver international : official journal of the International Association for the Study of the Liver

Sasaki M,Miyakoshi M,Sato Y,Nakanuma Y

MA1-19162 was used in western blot to investigate autophagy and senescence in primary biliary cirrhosis.

Thu Mar 01 00:00:00 EST 2012

Increased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cells.

Experimental cell research

Tai CJ,Shen SC,Lee WR,Liao CF,Deng WP,Chiou HY,Hsieh CI,Tung JN,Chen CS,Chiou JF,Li LT,Lin CY,Hsu CH,Jiang MC

MA1-19162 was used in immunocytochemistry, immunoprecipitation, and western blot to report that CSE1L regulates the association of alpha-tubulin with beta-tubulin and promotes migration of MCF-7 breast cancer cells

Fri Oct 15 00:00:00 EDT 2010

Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon.

Molecular and cellular biochemistry

Tsao TY,Tsai CS,Tung JN,Chen SL,Yue CH,Liao CF,Wang CC,Jiang MC

MA1-19162 was used in western blot to study the regulatory role of CSE1L/CAS in the secretion of human colorectal cells

Wed Jul 01 00:00:00 EDT 2009

Polycomb group protein Bmi1 is overexpressed and essential in anchorage-independent colony formation, cell proliferation and repression of cellular senescence in cholangiocarcinoma: tissue and culture studies.

Human pathology

Sasaki M,Yamaguchi J,Ikeda H,Itatsu K,Nakanuma Y

MA1-19162 was used in western blot to elucidate the role of Bmi1 expression in intrahepatic cholangiocarcinoma.

Tue Dec 01 00:00:00 EST 2009

TRIM36 interacts with the kinetochore protein CENP-H and delays cell cycle progression.

Biochemical and biophysical research communications

Miyajima N,Maruyama S,Nonomura K,Hatakeyama S

MA1-19162 was used in immunocytochemistry to study the interaction of TRIM36 with centromere protein-H

Fri Apr 10 00:00:00 EDT 2009

Tripartite motif protein 32 facilitates cell growth and migration via degradation of Abl-interactor 2.

Cancer research

Kano S,Miyajima N,Fukuda S,Hatakeyama S

MA1-19162 was used in western blot to determine the contribution of TRIM32 to carcinogenesis

Tue Jul 15 00:00:00 EDT 2008

Stat3 promotes metastatic progression of prostate cancer.

The American journal of pathology

Abdulghani J,Gu L,Dagvadorj A,Lutz J,Leiby B,Bonuccelli G,Lisanti MP,Zellweger T,Alanen K,Mirtti T,Visakorpi T,Bubendorf L,Nevalainen MT

MA1-19162 was used in immunocytochemistry to test if Stat3 stimulates metastatic progression of prostate cancer

Sun Jun 01 00:00:00 EDT 2008

The overexpression of polycomb group proteins Bmi1 and EZH2 is associated with the progression and aggressive biological behavior of hepatocellular carcinoma.

Laboratory investigation; a journal of technical methods and pathology

Sasaki M,Ikeda H,Itatsu K,Yamaguchi J,Sawada S,Minato H,Ohta T,Nakanuma Y

MA1-19162 was used in western blot to study Bmi1 and EZH2 expression in hepatocellular carcinoma and dysplastic nodules

Fri Aug 01 00:00:00 EDT 2008

The X11L/X11beta/MINT2 and X11L2/X11gamma/MINT3 scaffold proteins shuttle between the nucleus and cytoplasm.

Experimental cell research

Sumioka A,Saito Y,Sakuma M,Araki Y,Yamamoto T,Suzuki T

MA1-19162 was used in immunohistochemistry to determine X11L and X11L2 nuclear export signal sequence and quantify protein recovery in nuclear fraction of mouse brain homogenates after leptomycin B treatment

Mon Mar 10 00:00:00 EDT 2008

CAS Enhances Chemotherapeutic Drug-Induced p53 Accumulation and Apoptosis: Use of CAS for High-Sensitivity Anticancer Drug Screening.

Toxicology mechanisms and methods

Liao CF,Luo SF,Tsai CS,Tsao TY,Chen SL,Jiang MC

MA1-19162 was used in western blot to report that CAS is an important p53 and apoptosis regulator and could be used for high-throughput anticancer drug screening

Tue Jan 01 00:00:00 EST 2008

Novel functions of ubiquitin ligase HRD1 with transmembrane and proline-rich domains.

Journal of pharmacological sciences

Omura T,Kaneko M,Onoguchi M,Koizumi S,Itami M,Ueyama M,Okuma Y,Nomura Y

MA1-19162 was used in western blot to determine the roles of human HRD1 domains

Sat Mar 01 00:00:00 EST 2008

A different pathway in the endoplasmic reticulum stress-induced expression of human HRD1 and SEL1 genes.

FEBS letters

Kaneko M,Yasui S,Niinuma Y,Arai K,Omura T,Okuma Y,Nomura Y

MA1-19162 was used in western blot to identify the pathways mediating ER stress-induced endoplasmic reticulum-associated degradation gene expression

Tue Nov 27 00:00:00 EST 2007

Role of APP phosphorylation in FE65-dependent gene transactivation mediated by AICD.

Genes to cells : devoted to molecular & cellular mechanisms

Nakaya T,Suzuki T

MA1-19162 was used in western blot to investigate the function of amyloid beta-protein precursor and phosphorylation of its intracellular domain fragment in the nuclear translocation of FE65

Thu Jun 01 00:00:00 EDT 2006

Mutant huntingtin impairs axonal trafficking in mammalian neurons in vivo and in vitro.

Molecular and cellular biology

Trushina E,Dyer RB,Badger JD,Ure D,Eide L,Tran DD,Vrieze BT,Legendre-Guillemin V,McPherson PS,Mandavilli BS,Van Houten B,Zeitlin S,McNiven M,Aebersold R,Hayden M,Parisi JE,Seeberg E,Dragatsis I,Doyle K,Bender A,Chacko C,McMurray CT

MA1-19162 was used in western blot to show that huntingtin is involved in fast axonal trafficking in mammals

Wed Sep 01 00:00:00 EDT 2004

Functional cooperation between interleukin-17 and tumor necrosis factor-alpha is mediated by CCAAT/enhancer-binding protein family members.

The Journal of biological chemistry

Ruddy MJ,Wong GC,Liu XK,Yamamoto H,Kasayama S,Kirkwood KL,Gaffen SL

MA1-19162 was used in immunocytochemistry to elucidate how IL-17 regulates IL-6.

Fri Jan 23 00:00:00 EST 2004

Mouse Fbw7/Sel-10/Cdc4 is required for notch degradation during vascular development.

The Journal of biological chemistry

Tsunematsu R,Nakayama K,Oike Y,Nishiyama M,Ishida N,Hatakeyama S,Bessho Y,Kageyama R,Suda T,Nakayama KI

MA1-19162 was used in western blot to study the role of Fbw7 in vascular development.

Fri Mar 05 00:00:00 EST 2004

Establishment of an oral squamous cell carcinoma cell line with high invasive and p27 degradation activities from a lymph node metastasis.

Oral oncology

Kudo Y,Kitajjma S,Sato S,Miyauchi M,Ogawa I,Takata T

MA1-19162 was used in western blot to characterize a new oral squamous cell carcinoma cell line

Tue Jul 01 00:00:00 EDT 2003

MSX2 promotes osteogenesis and suppresses adipogenic differentiation of multipotent mesenchymal progenitors.

The Journal of biological chemistry

Cheng SL,Shao JS,Charlton-Kachigian N,Loewy AP,Towler DA

MA1-19162 was used in western blot to study BMP2-Msx2 signaling in vascular calcification.

Fri Nov 14 00:00:00 EST 2003

Soluble tubulin complexes in oocytes of the common leopard frog, Rana pipiens, contain gamma-tubulin.

Molecular reproduction and development

Lessman CA,Kim H

MA1-19162 was used in immunoprecipitation to characterize tubulin complexes using frog oocytes.

Sat Sep 01 00:00:00 EDT 2001

Neuron-specific phosphorylation of Alzheimer's beta-amyloid precursor protein by cyclin-dependent kinase 5.

Journal of neurochemistry

Iijima K,Ando K,Takeda S,Satoh Y,Seki T,Itohara S,Greengard P,Kirino Y,Nairn AC,Suzuki T

MA1-19162 was used in immunocytochemistry to examine how phosphorylation of APP by Cdk5 alters its localization.

Fri Sep 01 00:00:00 EDT 2000

Role of phosphorylation of Alzheimer's amyloid precursor protein during neuronal differentiation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ando K,Oishi M,Takeda S,Iijima K,Isohara T,Nairn AC,Kirino Y,Greengard P,Suzuki T

MA1-19162 was used in immunocytochemistry to study the phosphorylated form of APP.

Tue Jun 01 00:00:00 EDT 1999

Bioinformatics

Protein Aliases: alpha tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; tubulin alpha 3; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2B class IIb; tubulin, beta polypeptide paralog

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Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; PMGYSA; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; TUBB2B

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UniProt ID: (Human) Q71U36, (Human) P68363, (Pig) Q2XVP4, (Human) Q9BQE3, (Human) P0DPH7, (Human) Q6PEY2, (Human) P68366, (Human) A6NHL2, (Human) Q9BVA1, (Mouse) Q5XJF8, (Mouse) P05213, (Mouse) P05216, (Mouse) P68368, (Mouse) Q3UX10, (Mouse) Q9CWF2

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Function(s)

GTPase activity structural molecule activity structural constituent of cytoskeleton protein binding GTP binding protein domain specific binding double-stranded RNA binding ubiquitin protein ligase binding molecular_function enzyme binding nucleotide binding tubulin

Process(es)

G2/M transition of mitotic cell cycle cytoskeleton organization microtubule-based process cytoskeleton-dependent intracellular transport cell division microtubule cytoskeleton organization cellular response to interleukin-4 biological_process platelet degranulation neuron migration protein polymerization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Tubulin Monoclonal Antibody (TU-01)

alpha Tubulin Antibody (MA1-19162) in ICC/IF

Product Details

MA1-19162

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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