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Invitrogen
Mouse IgG2a Isotype Control (MOPC-173), DyLight™ 650
FIGURE: 1 / 3
Mouse IgG2a Isotype Control (MA1-193-D650) in ICC/IF
Imunofluorescent analysis of Lin28 (red) in Human episomal iPSC cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with Dylight 650-conjugated Lin28 monoclonal antibody (right, Product # MA1-016-D650) or Dylight 650-conjugated Mouse IgG2a... View More
Product Details
MA1-193-D650
Applications
Tested Dilution
Western Blot (WB)
Assay-dependent
Immunohistochemistry (Paraffin) (IHC (P))
Assay-dependent
Immunohistochemistry (Frozen) (IHC (F))
Assay-dependent
Immunocytochemistry (ICC/IF)
Assay-dependent
Flow Cytometry (Flow)
Assay-dependent
Control (Ctrl)
Assay-dependent
Product Specifications
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Isotype Control
Clone
MOPC-173
Immunogen
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice
Conjugate
Excitation/Emission Max
651/673 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536961
Product Specific Information
This antibody reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
MA1-193-D650 can be used as a negative control in any immunoassay where a DyLight 650-conjugated primary antibody of the mouse IgG2a isotype is used. DyLight 650 has an excitation/emission of 652/672nm.
Target Information
Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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