Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
HLA-G Monoclonal Antibody (MEM-G/9), FITC
FIGURE: 1 / 4
HLA-G Antibody (MA1-19591) in Flow
Flow Cytometry analysis of HLA-G using HLA-G Monoclonal Antibody (MEM-G/9), FITC (Product # MA1-19591). Separation of HLA-G transfected LCL cells stained using anti-human HLA-G (MEM-G/9) FITC antibody (concentration in sample 1 μg/mL, red-filled) from HLA-G transfected LCL cells stained using mouse IgG1 isotype control FITC antibody (concentration in sample 1 μg/mL, same as HLA-G FITC concentration, black-dashed) in flow cytometry analysis (surface staining) of suspension of HLA-G transfected LCL cells.
Product Details
MA1-19591
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MEM-G/9
Immunogen
Recombinant human HLA-G refolded with beta- 2 Microglobulin and peptide.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1076722
Product Specific Information
This antibody reacts with an extracellular epitope on native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported.
It will not cross-react with mouse.
Target Information
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: b2 microglobulin; DADB-15K14.8; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G antigen; HLA-G histocompatibility antigen, class I, G; MHC class I antigen G
Gene Aliases: HLA-6.0; HLA-G; HLAG; MHC-G
UniProt ID: (Human) P17693
Entrez Gene ID: (Human) 3135
antigen processing and presentation of peptide antigen via MHC class I
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent
positive regulation of tolerance induction
positive regulation of T cell tolerance induction
immune response-inhibiting cell surface receptor signaling pathway
cellular defense response
antigen processing and presentation
positive regulation of interleukin-12 production
negative regulation of T cell proliferation
positive regulation of regulatory T cell differentiation
regulation of immune response
negative regulation of immune response
interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
negative regulation of dendritic cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support