Invitrogen

CD62L Monoclonal Antibody (LT-TD180), APC

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Reference

View all (161) L-Selectin (CD62L) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Advanced Verification (1)

FIGURE: 1 / 3

CD62L Antibody (MA1-19715) in Flow

Separation of human CD62L positive lymphocytes (red-filled) from thrombocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (LT-TD180) APC Monoclonal antibody (Product # MA1-19715) using a dilution of 10 µL reagent/100 µL of peripheral whole blood.

Product Image Gallery

CD62L Antibody — FIG 1 HIV-1 infection downregulates cell surface CD62L on CD4 + T lymphocytes. (A and B) Primary CD4 + T lymphocytes were infected with HIV-1 (VSV-G-pseudotyped strain NL4-3) or not infected (n.i.), cultivated for 5 days (d), activated with SEB or not activated (n.a.), and analyzed 3 days later by flow cytometry for cell surface CD62L and intracellular HIV-1 p24 expression. (A) The filled histograms show CD62L fluorescence on cells negative for p24 expression or expressing low p24 levels (p24 neg/low ) and p24 pos cells; control IgG (open histograms) and CD62L MFI are also shown. FSC, forward scatter. (B to D) Scatter dot plots depicting mean (+-SEM) CD62L MFI from 4 experiments like the one shown in panel A (B), from 6 experiments in which SEB activation was performed immediately after infection and FACS analysis was performed 5 days later (C), and from 6 experiments in which cells were infected 3 days after activation with PHA and analyzed after an additional 7 days (D). (E) CD4 + T cells were infected and analyzed as for panel A with HIV-1 strain NL4-3 or SF2 or HIV-1 NL4-3 that was not pseudotyped, either untreated, heat inactivated (60degC), or treated with PI during virus production. The bars indicate the CD62L MFI (mean and SEM) of noninfected (white and gray) and p24 pos (black) cells in 6 experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 by one-way ANOVA. {TM}

Product Details

MA1-19715

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

10 µL x 100 µL whole blood or 10^6 cells

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

LT-TD180

Immunogen

Peripheral blood leukocytes.

View immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1073226

Product Specific Information

This antibody reacts with an extracellular epitope of CD62L (L-selectin), a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.

Target Information

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

HIV-1 Nef and Vpu Interfere with L-Selectin (CD62L) Cell Surface Expression To Inhibit Adhesion and Signaling in Infected CD4+ T Lymphocytes.

Journal of virology

Vassena L,Giuliani E,Koppensteiner H,Bolduan S,Schindler M,Doria M

MA1-19715 was used in flow cytometry to study dysregulation of CD62L expression during HIV infection.

Fri May 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD62 antigen-like family member L; CD62L; gp90-MEL; hLHRc; L-selectin; LAM-1; LECAM1; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; TQ1

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Gene Aliases: CD62L; LAM1; LECAM1; LEU8; LNHR; LSEL; LYAM1; PLNHR; SELL; TQ1

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UniProt ID: (Human) P14151

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Entrez Gene ID: (Human) 6402

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Function(s)

protease binding protein binding heparin binding carbohydrate binding glycosphingolipid binding cell adhesion molecule binding

Process(es)

cell adhesion response to ATP regulation of immune response leukocyte migration

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD62L Monoclonal Antibody (LT-TD180), APC

CD62L Antibody (MA1-19715) in Flow

Product Details

MA1-19715

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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