Invitrogen

6x-His Tag Monoclonal Antibody (HIS.H8), Biotin

6x-His Tag Antibody in Western Blot (WB)

FIGURE: 1 / 12

6x-His Tag Antibody (MA1-21315-BTIN) in WB

Western Blot was performed using Anti-6x-His Tag Monoclonal Antibody (HIS.H8), Biotin (Product # MA1-21315-BTIN) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 60 µg (Lane 1), empty vector control, 60 µg (Lane 2), His-p65-YFP, 60 µg (Lane 3), His-p65-YFP, 40 µg (Lane 4), His-p65-YFP, 20 µg (Lane 5); HA-H3-FLAG, 60 µg (Lane 6) and 25 ng of Positope (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferre... View More

6x-His Tag Antibody in Immunocytochemistry (ICC/IF)

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Product Details

MA1-21315-BTIN

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:500-1:2,000

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Fluorescence Resonance Energy Transfer (FRET)

- -

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

HIS.H8

Immunogen

6x His synthetic peptide.

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with proprietary stabilizer

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536983

Product Specific Information

MA1-21315-BTIN has been successfully used for Western blot, ELISA, IF and IP

Target Information

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rare, convergent antibodies targeting the stem helix broadly neutralize diverse betacoronaviruses.

Cell host & microbe

Dacon C,Peng L,Lin TH,Tucker C,Lee CD,Cong Y,Wang L,Purser L,Cooper AJR,Williams JK,Pyo CW,Yuan M,Kosik I,Hu Z,Zhao M,Mohan D,Peterson M,Skinner J,Dixit S,Kollins E,Huzella L,Perry D,Byrum R,Lembirik S,Murphy M,Zhang Y,Yang ES,Chen M,Leung K,Weinberg RS,Pegu A,Geraghty DE,Davidson E,Doranz BJ,Douagi I,Moir S,Yewdell JW,Schmaljohn C,Crompton PD,Mascola JR,Holbrook MR,Nemazee D,Wilson IA,Tan J

MA1-21315-BTIN was used in ELISA, Immunocytochemistry, Immunoprecipitation, Western Blot, Sample Prep to identify a class of IGHV1-46/IGKV3-20 antibodies that broadly neutralize betacoronaviruses by targeting the stem helix but indicate these antibodies constitute a small fraction of the broadly reactive antibody response to betacoronaviruses after SARS-CoV-2 infection.

Wed Jan 11 00:00:00 EST 2023

Voltage sensor dynamics of a bacterial voltage-gated sodium channel NavAb reveal three conformational states.

The Journal of biological chemistry

Han S,Vance J,Jones S,DeCata J,Tran K,Cummings J,Wang S

MA1-21315-BTIN was used in Fluorescence Resonance Energy Transfer to conduct both H+ and Na+ and were blocked by the local anesthetic lidocaine.

Wed Mar 01 00:00:00 EST 2023

Cell surface glycan engineering reveals that matriglycan alone can recapitulate dystroglycan binding and function.

Nature communications

Sheikh MO,Capicciotti CJ,Liu L,Praissman J,Ding D,Mead DG,Brindley MA,Willer T,Campbell KP,Moremen KW,Wells L,Boons GJ

MA1-21315-BTIN was used in Functional assays to indicate that matriglycan alone is necessary and sufficient for IIH6 staining, Laminin and LASV GP1 binding, and Lassa-pseudovirus infection and support a model in which it is a tunable receptor for which increasing chain length enhances ligand-binding capacity.

Fri Jun 24 00:00:00 EDT 2022

Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses.

eLife

Wang L,Johnson ZL,Wasserman MR,Levring J,Chen J,Liu S

MA1-21315-BTIN was used in Immunocytochemistry-immunoflourescence to investigate the conformational changes of bovine MRP1 (bMRP1) in real time using a single-molecule fluorescence spectroscopy.

Wed May 27 00:00:00 EDT 2020

A novel monoclonal antibody targeting carboxymethyllysine, an advanced glycation end product in atherosclerosis and pancreatic cancer.

PloS one

Wendel U,Persson N,Risinger C,Bengtsson E,Nodin B,Danielsson L,Welinder C,Nordin Fredrikson G,Jansson B,Blixt O

MA1-21315-BTIN was used in Immunohistochemistry to demonstrate a promising methodology for high-throughput generation of epitope-mapped monoclonal antibodies against advanced glycation end products.

Mon Mar 19 00:00:00 EDT 2018

Microtubule minus-end aster organization is driven by processive HSET-tubulin clusters.

Nature communications

Norris SR,Jung S,Singh P,Strothman CE,Erwin AL,Ohi MD,Zanic M,Ohi R

MA1-21315-BTIN was used to study microtubule aster formation by human minus-end-directed kinesin-14.

Mon Jul 09 00:00:00 EDT 2018

Programmable assembly of pressure sensors using pattern-forming bacteria.

Nature biotechnology

Cao Y,Feng Y,Ryser MD,Zhu K,Herschlag G,Cao C,Marusak K,Zauscher S,You L

MA1-21315-BTIN was used in Immunocytochemistry to demonstrate programmable fabrication of three-dimensional materials by printing engineered, self-patterning bacteria on permeable membranes.

Wed Nov 01 00:00:00 EDT 2017

Conservative site-specific and single-copy transgenesis in human LINE-1 elements.

Nucleic acids research

Vijaya Chandra SH,Makhija H,Peter S,Myint Wai CM,Li J,Zhu J,Ren Z,D'Alcontres MS,Siau JW,Chee S,Ghadessy FJ,Dröge P

Published figure using 6x-His Tag monoclonal antibody (Product # MA1-21315-BTIN) in Western Blot

Thu Apr 07 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: 6His tag; 6X His; His Tag; Histidine Tag

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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