Invitrogen

NK1.1 Monoclonal Antibody (PK136)

NK1.1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 2

NK1.1 Antibody (MA1-70100) in IHC (P)

Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded mouse spleen sections at 2.5x (Left) and 40x (Right) magnification. Primary antibody was a NK cells monoclonal antibody (clone PK136) and was at a dilution of 1:100; followed by staining with DAB for 1 minute.

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Product Details

MA1-70100

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/1 million cells

View 6 publications 6 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

PK136

Immunogen

NK-1+ cells from mouse spleen and bone marrow

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2296673

Product Specific Information

Clone PK136 is specific for mouse NK1.1, also known as NKR-PIC and Ly-55. Mouse NK1.1 is expressed on NK cells and NKT cells on the following strains: C57BL, FVB/N and NZB. There are published reports that PK136 also binds to NKR-PIB on SJL/K NK cells.

In addition to Flow and IHC, this clone has also been reported to work for in-vitro and in-vivo depletion assays.

Target Information

NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161, cluster of differentiation 161), refers to Natural Killer (NK) cells, lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus. NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Local and Abscopal Effect Observed with Liposomal Encapsulation of Intratumorally Injected Oncolytic Adenoviral Therapy.

Cancers

Dong T,Shah JR,Phung AT,Larson C,Sanchez AB,Aisagbonhi O,Blair SL,Oronsky B,Trogler WC,Reid T,Kummel AC

MA1-70100 was used in Immunohistochemistry-immunofluorescence to evaluate the in vivo therapeutic efficacy of oncolytic serotype 5 adenovirus TAV255 in CAR-deficient tumors.

Mon Jun 12 00:00:00 EDT 2023

Role of PCIF1-mediated 5'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy.

The EMBO journal

Wang L,Wu L,Zhu Z,Zhang Q,Li W,Gonzalez GM,Wang Y,Rana TM

MA1-70100 was used in Flow Cytometry, Immunohistochemistry (Paraffin) to provide a proof-of-concept for tumor growth suppression using LNP-CMsiRNA to silence target genes in cancer.

Mon Jan 16 00:00:00 EST 2023

Low-Dose Radiotherapy Reverses Tumor Immune Desertification and Resistance to Immunotherapy.

Cancer discovery

Herrera FG,Ronet C,Ochoa de Olza M,Barras D,Crespo I,Andreatta M,Corria-Osorio J,Spill A,Benedetti F,Genolet R,Orcurto A,Imbimbo M,Ghisoni E,Navarro Rodrigo B,Berthold DR,Sarivalasis A,Zaman K,Duran R,Dromain C,Prior J,Schaefer N,Bourhis J,Dimopoulou G,Tsourti Z,Messemaker M,Smith T,Warren SE,Foukas P,Rusakiewicz S,Pittet MJ,Zimmermann S,Sempoux C,Dafni U,Harari A,Kandalaft LE,Carmona SJ,Dangaj Laniti D,Irving M,Coukos G

MA1-70100 was used in Immunohistochemistry (Paraffin) to report that low-dose radiotherapy (LDRT) of murine tumors promotes T-cell infiltration and enables responsiveness to combinatorial immunotherapy in an IFN-dependent manner.

Sat Jan 01 00:00:00 EST 2022

Targeted gene silencing in the nervous system with CRISPR-Cas13.

Science advances

Powell JE,Lim CKW,Krishnan R,McCallister TX,Saporito-Magriña C,Zeballos MA,McPheron GD,Gaj T

MA1-70100 was used in Immunohistochemistry to demonstrate that RfxCas13d, a Cas13 ortholog with favorable characteristics to other family members, can be delivered to the mouse spinal cord and brain to silence neurodegeneration-associated genes.

Fri Jan 21 00:00:00 EST 2022

Netrin G1 Promotes Pancreatic Tumorigenesis through Cancer-Associated Fibroblast-Driven Nutritional Support and Immunosuppression.

Cancer discovery

Francescone R,Barbosa Vendramini-Costa D,Franco-Barraza J,Wagner J,Muir A,Lau AN,Gabitova L,Pazina T,Gupta S,Luong T,Rollins D,Malik R,Thapa RJ,Restifo D,Zhou Y,Cai KQ,Hensley HH,Tan Y,Kruger WD,Devarajan K,Balachandran S,Klein-Szanto AJ,Wang H,El-Deiry WS,Vander Heiden MG,Peri S,Campbell KS,Astsaturov I,Cukierman E

MA1-70100 was used in Immunohistochemistry to identify Netrin G1 (NetG1) as a promoter of PDAC tumorigenesis.

Mon Feb 01 00:00:00 EST 2021

Exercise training improves radiotherapy efficiency in a murine model of prostate cancer.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Dufresne S,Guéritat J,Chiavassa S,Noblet C,Assi M,Rioux-Leclercq N,Rannou-Bekono F,Lefeuvre-Orfila L,Paris F,Rébillard A

MA1-70100 was used in Flow Cytometry, Immunohistochemistry (Paraffin) to show that (a) ET enhances RT efficiency by potentiating NK cell infiltration, and (b) while ET alone and ET combined with RT both reduce tumor growth, the mechanisms mediating these effects are different.

Wed Apr 01 00:00:00 EDT 2020

Increased FGF-23 levels are linked to ineffective erythropoiesis and impaired bone mineralization in myelodysplastic syndromes.

JCI insight

Weidner H,Baschant U,Lademann F,Ledesma Colunga MG,Balaian E,Hofbauer C,Misof BM,Roschger P,Blouin S,Richards WG,Platzbecker U,Hofbauer LC,Rauner M

MA1-70100 was used in Flow Cytometry to identify FGF-23 as a link between altered bone structure and ineffective erythropoiesis in MDS with the prospects of a targeted therapeutic intervention.

Thu Aug 06 00:00:00 EDT 2020

Combined α-programmed death-1 monoclonal antibody blockade and fractionated radiation therapy reduces tumor growth in mouse EL4 lymphoma.

Cancer biology & therapy

Lin X,Zeng T,Xiong J,Zhang Q,Jiang P,Li X,Lin S,Xu Q,Weng H,Lai H,Gong H,Lin J,Cheng N,Tian X,Xu Y,Fang S,Jin R,Chen Z,Yang J,Morton L,Yueh B,Lin J

MA1-70100 was used in Immunohistochemistry to examine the effects of combined α-programmed death-1 monoclonal antibody immunotherapy with fractionated radiotherapy on antitumour immuntiy and tumour growth in lymphoma.

Mon Jul 06 00:00:00 EDT 2020

Intravesical treatment of chemotherapeutic agents sensitizes bacillus Calmette‑Guerin by the modulation of the tumor immune environment.

Oncology reports

Hori S,Miyake M,Tatsumi Y,Morizawa Y,Nakai Y,Onishi S,Onishi K,Iida K,Gotoh D,Itami Y,Tanaka N,Fujimoto K

MA1-70100 was used in Immunohistochemistry to show that systemic changes in IL-17 and G-CSF may be involved in topical immunomodulation.

Fri Mar 01 00:00:00 EST 2019

A novel Cd8-cis-regulatory element preferentially directs expression in CD44hiCD62L+ CD8+ T cells and in CD8αα+ dendritic cells.

Journal of leukocyte biology

Sakaguchi S,Hombauer M,Hassan H,Tanaka H,Yasmin N,Naoe Y,Bilic I,Moser MA,Hainberger D,Mayer H,Seiser C,Bergthaler A,Taniuchi I,Ellmeier W

MA1-70100 was used in ChIP assay to identify Cd8 cis-regulatory elements

Wed Apr 01 00:00:00 EDT 2015

Long-term, multilineage hematopoiesis occurs in the combined absence of beta-catenin and gamma-catenin.

Blood

Jeannet G,Scheller M,Scarpellino L,Duboux S,Gardiol N,Back J,Kuttler F,Malanchi I,Birchmeier W,Leutz A,Huelsken J,Held W

MA1-70100 was used in flow cytometry to investigate whether hematopoietic cells transduce canonical Wnt signals in the absence of beta- and gamma-catenin

Tue Jan 01 00:00:00 EST 2008

MHC class I molecules on adenovirus E1A-expressing tumor cells inhibit NK cell killing but not NK cell-mediated tumor rejection.

International immunology

Routes JM,Ryan JC,Ryan S,Nakamura M

MA1-70100 was used in flow cytometry to investigate the capacity of E1A to sensitize cells to multiple NK cell killing mechanisms

Mon Oct 01 00:00:00 EDT 2001

Mouse NKR-P1B, a novel NK1.1 antigen with inhibitory function.

Journal of immunology (Baltimore, Md. : 1950)

Carlyle JR,Martin A,Mehra A,Attisano L,Tsui FW,Zúñiga-Pflücker JC

MA1-70100 was used in flow cytometry and immunoprecipitation to identify NKR-P1B as a NK1.1 antigen

Sat May 15 00:00:00 EDT 1999

Natural killer cell proliferation induced by anti-NK1.1 and IL-2.

Immunology and cell biology

Reichlin A,Yokoyama WM

MA1-70100 was used in flow cytometry to report that immobilized anti-NK1.1 triggers only a subpopulation of NK cells to kill

Wed Apr 01 00:00:00 EST 1998

Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen.

Hybridoma

Sentman CL,Hackett J,Moore TA,Tutt MM,Bennett M,Kumar V

MA1-70100 was used in flow cytometry to describe three new NK specific monoclonal antibodies

Fri Dec 01 00:00:00 EST 1989

Bioinformatics

Protein Aliases: CD161 antigen-like family member B; CD161 antigen-like family member C; CD161b; CD161c; Inhibitory receptor NKR-P1B; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1B allele A; Killer cell lectin-like receptor subfamily B member 1B allele B; Killer cell lectin-like receptor subfamily B member 1C; killer cell lectin-like receptor subfamily B member 1D; KLRB1; Ly-55; ly-55b; Ly-55c; Ly-55d; Ly55; lymphocyte antigen 55 complex, locus B; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 55 complex, locus D; lymphocyte antigen 55b; Lymphocyte antigen 55c; Lymphocyte antigen 55d; lymphocyte antigen 59; MGC163757; MGC163759; natural killer cell receptor protein NKR-P1B; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; Natural killer cell surface protein NKR-P1B allele B6; natural killer cell surface protein NKR-P1B allele SJL/BALB; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; natural killer cells; nk cells; NK1.1; NKR-P1 34; NKR-P1 40; NKR-P1.9; NKR-P1C; NKR-P1D

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Gene Aliases: AI462337; CD161; Klrb1b; Klrb1c; Klrb1d; ly-55c; Ly-59; Ly55b; Ly55c; Ly55d; Ly59; Nk-1; Nk-1.2; NK-RP1; Nk1; NK1.1; Nk1.2; NKR-P1.9; NKR-P1B; NKR-P1D; NKRP1; Nkrp1-b; NKRP140; Nkrp1b; Nkrp1c; Nkrp1d

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UniProt ID: (Mouse) Q99JB4, (Mouse) P27814

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Entrez Gene ID: (Mouse) 80782, (Mouse) 17059

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Function(s)

receptor activity protein binding carbohydrate binding protein homodimerization activity transmembrane signal receptor

Process(es)

negative regulation of natural killer cell mediated cytotoxicity natural killer cell activation positive regulation of natural killer cell mediated cytotoxicity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NK1.1 Monoclonal Antibody (PK136)

NK1.1 Antibody (MA1-70100) in IHC (P)

Product Details

MA1-70100

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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