Invitrogen

Calprotectin (S100A9) Monoclonal Antibody (MAC387), FITC

Product Details

MA1-80263

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Flow Cytometry (Flow)

Neat

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Deer, Horse, Cat, Guinea pig, Goat, Human, Non-human primate, Pig, Rabbit, Rat

Published species

Bovine, Dog, Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MAC387

Immunogen

Human monocytes.

View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1% BSA

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2184276

Product Specific Information

This clone recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains (Flavell et al. 1987) subsequent studies indicate that the antibody detects an epitope exclusively expressed on the beta chain (Goebeler et al. 1994) demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha. The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cellds in a wide range of species.

Membrane permeabilization is required for flow cytometry applications. Use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Short-term safety and efficacy of the biodegradable iron stent in mini-swine coronary arteries.

Chinese medical journal

Wu C,Qiu H,Hu XY,Ruan YM,Tian Y,Chu Y,Xu XL,Xu L,Tang Y,Gao RL

MA1-80263 was used in immunohistochemistry to use a mini-swine model to study the safety and efficacy of a coronary artery biodegradable iron stent

Wed Jun 25 00:00:00 EDT 2014

Xenotransplantation of human unrestricted somatic stem cells in a pig model of acute myocardial infarction.

Xenotransplantation

Gahremanpour A,Vela D,Zheng Y,Silva GV,Fodor W,Cardoso CO,Baimbridge F,Fernandes MR,Buja LM,Perin EC

MA1-80263 was used in immunohistochemistry to study whether xenotransplantation with human CD34-/CD45- unrestricted somatic stem cells is of therapeutic benefit in a porcine model of acute myocardial infarction

Mon Sep 30 00:00:00 EDT 2013

Transplantation with autologous mesenchymal stem cells after acute myocardial infarction evaluated by magnetic resonance imaging: an experimental study.

Journal of thoracic imaging

Lu M,Zhao S,Liu Q,Jiang S,Song P,Qian H,Zhang Y,Ling J,Yan C,Cheng H,Ma N,Zhao H,Liu Y

MA1-80263 was used in immunohistochemistry to study the effects of transplantation with autologous mesenchymal stem cells in postinfarction swine hearts

Thu Mar 01 00:00:00 EST 2012

Gene expression profile of vascular endothelial growth factor (VEGF) and its receptors in various cell types of the canine lymph node using laser capture microdissection (LCM).

Veterinary immunology and immunopathology

Jais A,Klein D,Wolfesberger B,Walter I

MA1-80263 was used in immunohistochemistry to study the expression of VEGF and its receptors in a range of cell types in normal canine lymph nodes.

Fri Apr 15 00:00:00 EDT 2011

The performance of photooxidatively crosslinked acellular bovine jugular vein conduits in the reconstruction of connections between pulmonary arteries and right ventricles.

Biomaterials

Lü WD,Zhang M,Wu ZS,Hu TH,Xu ZJ,Liu W,Hu YR

MA1-80263 was used in immunohistochemistry to evaluate photooxidatively crosslinked acellular bovine jugular vein conduits in vascular reconstruction

Thu Apr 01 00:00:00 EDT 2010

Insulin increases resistance to burn wound infection-associated sepsis.

Critical care medicine

Gauglitz GG,Toliver-Kinsky TE,Williams FN,Song J,Cui W,Herndon DN,Jeschke MG

MA1-80263 was used in flow cytometry to investigate the therapeutic efficacy of insulin on the treatment of sepsis

Fri Jan 01 00:00:00 EST 2010

Chronic intake of a high-cholesterol diet resulted in hepatic steatosis, focal nodular hyperplasia and fibrosis in non-obese mice.

The British journal of nutrition

Sumiyoshi M,Sakanaka M,Kimura Y

MA1-80263 was used in immunohistochemistry to investigate the influence of high-cholesterol food on liver function in normal mice

Mon Feb 01 00:00:00 EST 2010

Characterization of a preclinical model of chronic ischemic wound.

Physiological genomics

Roy S,Biswas S,Khanna S,Gordillo G,Bergdall V,Green J,Marsh CB,Gould LJ,Sen CK

MA1-80263 was used in immunohistochemistry to develop and evaluate a preclinical porcine model of chronic ischemic wound

Wed May 13 00:00:00 EDT 2009

Role of cardiopulmonary bypass and arrested heart status in the early cell distribution after intracoronary infusion of bone marrow stromal cells.

The Journal of surgical research

Song P,Zhang H,Lu MJ,Li J,Liu XW,Wei YJ,Hu SS

MA1-80263 was used in immunohistochemistry to study the distribution of cells following intracoronary infusion of bone marrow stromal cells

Fri May 01 00:00:00 EDT 2009

Injection of bone marrow mesenchymal stem cells in the borderline area of infarcted myocardium: heart status and cell distribution.

The Journal of thoracic and cardiovascular surgery

Zhang H,Song P,Tang Y,Zhang XL,Zhao SH,Wei YJ,Hu SS

MA1-80263 was used in immunohistochemistry to study the distribution of bone marrow mesenchymal stem cells following injection into infarcted myocardium

Thu Nov 01 00:00:00 EDT 2007

Histopathology of spleen allograft rejection in miniature swine.

International journal of experimental pathology

Dor FJ,Gollackner B,Kuwaki K,Ko DS,Cooper DK,Houser SL

MA1-80263 was used in immunohistochemistry to study the histopathology of spleen allograft rejection in miniature swine

Tue Feb 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: AI323541; B8Ag; BEE11; BEE22; calcium binding protein; S100 protein; calgranulin A; calgranulin B; Calgranulin-A; Calgranulin-B; calprotectin; Calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; Cystic fibrosis antigen; intracellular calcium-binding protein (MRP14); Leukocyte L1 complex heavy chain; Leukocyte L1 complex light chain; macrophage migration inhibitory factor-related protein-14; macrophage migration inhibitory factor-related protein-8; Migration inhibitory factor-related protein 14; Migration inhibitory factor-related protein 8; MRP-14; MRP-8; Myeloid-related protein 14; Neutrophil cytosolic 23 kDa protein; Neutrophil cytosolic 7 kDa protein; p14; p23; P7; p8; Protein S100-A8; Protein S100-A9; S100 calcium binding protein A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100A8/S100A9; Urinary stone protein band A

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Gene Aliases: 60B8AG; CAGA; CAGB; CFAG; CGLA; CGLB; CP-10; L1AG; LIAG; MA387; MAC387; MIF; MRP-14; MRP-8; MRP14; MRP8; NIF; P14; P8; S100A8; S100A9

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UniProt ID: (Bovine) P28782, (Human) P05109, (Bovine) P28783, (Rabbit) P50117, (Human) P06702, (Rat) P50115, (Rat) P50116

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Function(s)

calcium ion binding microtubule binding zinc ion binding antioxidant activity Toll-like receptor 4 binding arachidonic acid binding RAGE receptor binding protein binding signal transducer activity molecular_function calmodulin-related

Process(es)

leukocyte migration involved in inflammatory response positive regulation of peptide secretion autophagy apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process astrocyte development peptidyl-cysteine S-nitrosylation neutrophil chemotaxis innate immune response positive regulation of inflammatory response neutrophil aggregation cellular oxidant detoxification positive regulation of intrinsic apoptotic signaling pathway cytokine production acute inflammatory response chronic inflammatory response inflammatory response response to zinc ion positive regulation of cell growth sequestering of zinc ion response to lipopolysaccharide chemokine production wound healing defense response to bacterium response to ethanol defense response to fungus positive regulation of NF-kappaB transcription factor activity regulation of cytoskeleton organization regulation of translation signal transduction cell-cell signaling positive regulation of blood coagulation actin cytoskeleton reorganization peptidyl-cysteine S-trans-nitrosylation regulation of integrin biosynthetic process leukocyte chemotaxis

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Calprotectin (S100A9) Monoclonal Antibody (MAC387), FITC

Product Details

MA1-80263

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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