Invitrogen

CD11b Monoclonal Antibody (M1/70.15), PE

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Datasheet

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Tech Support

Product Details

MA1-80486

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Neat-1:10

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 15 publications 15 publications

Product Specifications

Species Reactivity

Human, Mouse, Rabbit

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

M1/70.15

Immunogen

T cells enriched splenocytes from B10 mice.

View immunogen

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 1% BSA, 5% sucrose

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_927452

Product Specific Information

Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Rat anti Mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Myeloid cell expansion elicited by the progression of spontaneous mammary carcinomas in c-erbB-2 transgenic BALB/c mice suppresses immune reactivity.

Blood

Melani C,Chiodoni C,Forni G,Colombo MP

MA1-80486 was used in flow cytometry to use the BALB-neuT model to study carcinogenesis.

Mon Sep 15 00:00:00 EDT 2003

Hematopoietic origin of glomerular mesangial cells.

Blood

Masuya M,Drake CJ,Fleming PA,Reilly CM,Zeng H,Hill WD,Martin-Studdard A,Hess DC,Ogawa M

MA1-80486 was used in flow cytometry to test if hematopoietic stem cells are capable of reconstituting mesangial cells.

Sat Mar 15 00:00:00 EST 2003

Passive transfer of flt-3L-derived dendritic cells delays diabetes development in NOD mice and associates with early production of interleukin (IL)-4 and IL-10 in the spleen of recipient mice.

Clinical and experimental immunology

Morin J,Faideau B,Gagnerault MC,Lepault F,Boitard C,Boudaly S

MA1-80486 was used in flow cytometry to assess the contribution of DC function in non-obese diabetic mice.

Mon Dec 01 00:00:00 EST 2003

Vascular cell adhesion molecule-1 (VCAM-1) blockade in collagen-induced arthritis reduces joint involvement and alters B cell trafficking.

Clinical and experimental immunology

Carter RA,Campbell IK,O'Donnel KL,Wicks IP

MA1-80486 was used in flow cytometry to investigate the role of VCAM-1 in collagen-induced arthritis.

Mon Apr 01 00:00:00 EST 2002

Long-term survival of corneal allografts is dependent on intact CD1d-reactive NKT cells.

Journal of immunology (Baltimore, Md. : 1950)

Sonoda KH,Taniguchi M,Stein-Streilein J

MA1-80486 was used in flow cytometry to test if the survival of a cornea graft is dependent on CD1d-reactive NKT cells.

Fri Feb 15 00:00:00 EST 2002

Bone marrow origin of hematopoietic progenitors and stem cells in murine muscle.

Blood

Kawada H,Ogawa M

MA1-80486 was used in flow cytometry to characterize skeletal muscle hematopoietic progenitors.

Mon Oct 01 00:00:00 EDT 2001

Severe inflammatory arthritis and lymphadenopathy in the absence of TNF.

The Journal of clinical investigation

Campbell IK,O'Donnell K,Lawlor KE,Wicks IP

MA1-80486 was used in flow cytometry to examine the impact of TNF on rheumatoid arthritis.

Fri Jun 01 00:00:00 EDT 2001

IL-12p40-dependent agonistic effects on the development of protective innate and adaptive immunity against Salmonella enteritidis.

Journal of immunology (Baltimore, Md. : 1950)

Lehmann J,Bellmann S,Werner C,Schröder R,Schütze N,Alber G

MA1-80486 was used in flow cytometry to investigate IL-12p40-dependent but IL-12p75-independent immune responses in mice infected with Salmonella enteritidis.

Thu Nov 01 00:00:00 EST 2001

Characterization of peripheral regulatory CD4+ T cells that prevent diabetes onset in nonobese diabetic mice.

Journal of immunology (Baltimore, Md. : 1950)

Lepault F,Gagnerault MC

MA1-80486 was used in flow cytometry to determine the phenotype and function of T cells that prevent diabetes.

Sat Jan 01 00:00:00 EST 2000

Acquired immunity to Chlamydia pneumoniae is dependent on gamma interferon in two mouse strains that initially differ in this respect after primary challenge.

Infection and immunity

Vuola JM,Puurula V,Anttila M,Mäkelä PH,Rautonen N

MA1-80486 was used in flow cytometry to examine the role of gamma interferon in a Chlamydia pneumoniae mouse model.

Tue Feb 01 00:00:00 EST 2000

CD1-reactive natural killer T cells are required for development of systemic tolerance through an immune-privileged site.

The Journal of experimental medicine

Sonoda KH,Exley M,Snapper S,Balk SP,Stein-Streilein J

MA1-80486 was used in flow cytometry to investigate the role of NKT cells in the development of tolerance.

Mon Nov 01 00:00:00 EST 1999

Identification of an early T cell progenitor for a pathway of T cell maturation in the bone marrow.

Proceedings of the National Academy of Sciences of the United States of America

Dejbakhsh-Jones S,Strober S

MA1-80486 was used in flow cytometry to identify a rare population of cells in the adult mouse bone marrow that generates CD4(+) and CD8(+) TCRalphabeta(+) T cells.

Tue Dec 07 00:00:00 EST 1999

Gene therapy of experimental autoimmune thyroiditis by in vivo administration of plasmid DNA coding for Fas ligand.

Journal of immunology (Baltimore, Md. : 1950)

Batteux F,Tourneur L,Trebeden H,Charreire J,Chiocchia G

MA1-80486 was used in flow cytometry to study FasL expression on thyrocytes.

Fri Jan 01 00:00:00 EST 1999

The IL-1 receptor-related T1 antigen is expressed on immature and mature mast cells and on fetal blood mast cell progenitors.

Journal of immunology (Baltimore, Md. : 1950)

Moritz DR,Rodewald HR,Gheyselinck J,Klemenz R

MA1-80486 was used in flow cytometry to assess the expression of the T1 surface antigen in murine hemopoietic organs.

Sun Nov 01 00:00:00 EST 1998

Exclusive development of T cell neoplasms in mice transplanted with bone marrow expressing activated Notch alleles.

The Journal of experimental medicine

Pear WS,Aster JC,Scott ML,Hasserjian RP,Soffer B,Sklar J,Baltimore D

MA1-80486 was used in flow cytometry to report that TAN1 is an oncoprotein.

Wed May 01 00:00:00 EDT 1996

Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations.

Journal of immunology (Baltimore, Md. : 1950)

Gagnerault MC,Touraine P,Savino W,Kelly PA,Dardenne M

MA1-80486 was used in flow cytometry to investigate the expression of prolactin receptor in lymphocyte subsets

Tue Jun 15 00:00:00 EDT 1993

Bioinformatics

Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); Leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit

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Gene Aliases: CD11B; CD11b/CD18; CR3; CR3A; F730045J24Rik; ITGAM; Ly-40; MAC-1; Mac-1a; MAC1; MAC1A; MO1A; SLEB6

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UniProt ID: (Human) P11215

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Entrez Gene ID: (Human) 3684, (Mouse) 16409, (Rabbit) 100351865

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Function(s)

glycoprotein binding protein binding metal ion binding opsonin binding heparin binding heparan sulfate proteoglycan binding protein heterodimerization activity integrin

Process(es)

cell adhesion integrin-mediated signaling pathway ectodermal cell differentiation extracellular matrix organization toll-like receptor 4 signaling pathway leukocyte migration leukocyte migration involved in inflammatory response leukocyte cell-cell adhesion microglia development single organismal cell-cell adhesion neutrophil chemotaxis cellular extravasation activated T cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD11b Monoclonal Antibody (M1/70.15), PE

Product Details

MA1-80486

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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