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CD32 Antibody (MA1-81209) in Flow
Flow cytometry analysis of CD32/FCGR2 was done on human peripheral blood granulocytes. Lysed whole blood was stained with a CD32/FCGR2 mouse monoclonal antibody (Product # MA1-81209) at a concentration of 0.2mg/mL for 1 hour at room temperature. Cells were washed twice with PBS, and stained with a Goat anti-Mouse IgG1 Secondary Antibody, RPE conjugate (Product # P-21129) at a dilution of 1:10 for 20 minutes at room temperature. A separate peripheral blood sample was also stained with a mouse IgG1 isotype control, RPE conjugate (Product # M... View More
Product Details
MA1-81209
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Non-human primate,
Pig
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
AT10
Immunogen
K562 cell line.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2103586
Product Specific Information
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is lymph node.
This product contains sodium azide; removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes are suitable for this purpose.
Target Information
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD32; CD32 receptor 2; CD32 receptor 2a; CDw32; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc fragment of IgG, low affinity IIc, receptor for (CD32); Fc gamma receptor II; Fc gamma receptor IIa; Fc gamma receptor IIb; Fc gamma receptor IIC; Fc gamma RIIb; Fc-gamma RII-a; Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma-RIIa; Fc-gamma-RIIb; Fc-gamma-RIIc; fcRII-a; fcRII-b; FGFR2B; IgG Fc receptor II-a; IgG Fc receptor II-b; IgG Fc receptor II-c; Immunoglobulin G Fc receptor II; immunoglobulin G Fc receptor II-c; Low affinity immunoglobulin gamma Fc region receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-b; Low affinity immunoglobulin gamma Fc region receptor II-c; MGC23887; MGC30032; RP11-5K23.6
Gene Aliases: CD32; CD32A; CD32B; CD32C; CDW32; FCG2; FcGR; FCGR2; FCGR2A; FCGR2A1; FCGR2B; FCGR2C; FCRIIC; IGFR2
UniProt ID: (Human) P12318, (Human) P31994, (Human) P31995
Entrez Gene ID: (Human) 2212, (Human) 2213, (Pig) 613131, (Human) 9103
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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