Invitrogen

PARP1 Monoclonal Antibody (C-2-10)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

10 References

View all (69) PARP1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

PARP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

PARP1 Antibody (MA3-950) in ICC/IF

Immunofluorescent analysis of PARP in HT-29 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a PARP monoclonal antibody (Product # MA3-950) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). PARP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

PARP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

PARP1 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of PARP using PARP Monoclonal Antibody (C-2-10) (Product # MA3-950) shows increased expression of Cleaved PARP in HeLa and Jurkat cell line upon Staurosporine treatment. {TM}

Product Details

MA3-950

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C-2-10

Immunogen

Purified calf thymus poly (ADP-ribose) polymerase.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325523

Product Specific Information

This antibody does not detect PARP from chicken tissues.

By Western blot, this antibody recognizes a 116 kDa protein which corresponds to PARP and the 85 kDa apoptosis-induced cleavage product of prICE and CPP32 from rat thymus extract.

This antibody recognizes an epitope found between amino acids 216-375 in the DNA-binding domain of PARP.

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prostate Cancer Cells Are Sensitive to Lysosomotropic Agent Siramesine through Generation Reactive Oxygen Species and in Combination with Tyrosine Kinase Inhibitors.

Cancers

Garcia EA,Bhatti I,Henson ES,Gibson SB

MA3-950 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to find that siramesine was the most effective lysosomotropic agent at inducing lysosome membrane permeabilization, increasing reactive oxygen species, and inducing cell death in three different prostate cancer cell lines.

Tue Nov 08 00:00:00 EST 2022

LRP1 Regulates Retinal Angiogenesis by Inhibiting PARP-1 Activity and Endothelial Cell Proliferation.

Arteriosclerosis, thrombosis, and vascular biology

Mao H,Lockyer P,Townley-Tilson WH,Xie L,Pi X

Published figure using PARP1 monoclonal antibody (Product # MA3-950) in Immunocytochemistry

Mon Feb 01 00:00:00 EST 2016

New insights into PARP inhibitors' effect on cell cycle and homology-directed DNA damage repair.

Molecular cancer therapeutics

Jelinic P,Levine DA

MA3-950 was used in western blot to study the off-target effects of the PARP inhibitors olaparib and veliparib on cell cycle progression and homology-directed DNA damage repair

Sun Jun 01 00:00:00 EDT 2014

Decreased parkin solubility is associated with impairment of autophagy in the nigrostriatum of sporadic Parkinson's disease.

Neuroscience

Lonskaya I,Hebron ML,Algarzae NK,Desforges N,Moussa CE

Published figure using PARP monoclonal antibody (Product # MA3-950) in Western Blot

Fri Mar 01 00:00:00 EST 2013

Identification of TSG101 functional domains and p21 loci required for TSG101-mediated p21 gene regulation.

PloS one

Lin YS,Chen YJ,Cohen SN,Cheng TH

MA3-950 was used in western blot to study the mechanisms underlying the regulation of p21 gene expression by TSG101

Mon Nov 24 00:00:00 EST 2014

ADP ribosylation by PARP-1 suppresses HOXB7 transcriptional activity.

PloS one

Wu X,Ellmann S,Rubin E,Gil M,Jin K,Han L,Chen H,Kwon EM,Guo J,Ha HC,Sukumar S

MA3-950 was used in western blot to investigate the interaction between HOXB7 and poly (ADP-ribose) polymerase-1

Wed Nov 28 00:00:00 EST 2012

Cellular dynamics of the negative transcription elongation factor NELF.

Experimental cell research

Yung TM,Narita T,Komori T,Yamaguchi Y,Handa H

MA3-950 was used in western blot to investigate the cellular localization and function of negative transcription elongation factor

Wed Jun 10 00:00:00 EDT 2009

Role of human transcription elongation factor DSIF in the suppression of senescence and apoptosis.

Genes to cells : devoted to molecular & cellular mechanisms

Komori T,Inukai N,Yamada T,Yamaguchi Y,Handa H

MA3-950 was used in western blot to investigate the role of human transcription elongation factor DSIF in the suppression of senescence and apoptosis

Sun Mar 01 00:00:00 EST 2009

Apoptosis-inducing factor is a major contributor to neuronal loss induced by neonatal cerebral hypoxia-ischemia.

Cell death and differentiation

Zhu C,Wang X,Huang Z,Qiu L,Xu F,Vahsen N,Nilsson M,Eriksson PS,Hagberg H,Culmsee C,Plesnila N,Kroemer G,Blomgren K

MA3-950 was used in western blot to characterize defects in harlequin mutant mice

Sun Apr 01 00:00:00 EDT 2007

Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs).

Oncogene

Duensing A,Medeiros F,McConarty B,Joseph NE,Panigrahy D,Singer S,Fletcher CD,Demetri GD,Fletcher JA

MA3-950 was used in western blot to identify pathways that are constitutively activated in a KIT-dependent manner in gastrointestinal stromal tumors

Thu May 13 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase 1; ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT 1; ARTD1; DNA ADP-ribosyltransferase PARP1; msPARP; NAD(+) ADP-ribosyltransferase 1; NAD(+) pADPRT-1; PARP-1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly ADP-ribose polymerase; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase PARP-1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; Protein poly-ADP-ribosyltransferase PARP1; unnamed protein product

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Gene Aliases: 5830444G22Rik; Adprp; ADPRT; ADPRT 1; ADPRT1; AI893648; ARTD1; C80510; I79_014850; pADPRT-1; PARP; PARP-1; PARP1; PPOL; sPARP-1

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UniProt ID: (Bovine) P18493, (Human) P09874, (Rat) P27008, (Chinese hamster) Q9R152

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Entrez Gene ID: (Bovine) 286764, (Human) 142, (Rat) 25591, (Chinese hamster) 100689463, (Mouse) 11545

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Function(s)

DNA binding zinc ion binding NAD binding protein ADP-ribosylase activity DNA ligase (ATP) activity NAD+ ADP-ribosyltransferase activity protein binding transcription factor binding enzyme binding protein kinase binding identical protein binding histone deacetylase binding poly(A) RNA binding protein N-terminus binding R-SMAD binding transferase activity, transferring pentosyl groups chromatin binding transferase activity transferase activity, transferring glycosyl groups metal ion binding DNA metabolism protein

Process(es)

double-strand break repair protein ADP-ribosylation cellular response to DNA damage stimulus positive regulation of cardiac muscle hypertrophy peptidyl-serine ADP-ribosylation peptidyl-glutamic acid poly-ADP-ribosylation DNA ADP-ribosylation protein poly-ADP-ribosylation protein auto-ADP-ribosylation positive regulation of double-strand break repair via homologous recombination ATP generation from poly-ADP-D-ribose negative regulation of transcription from RNA polymerase II promoter nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding double-strand break repair via homologous recombination lagging strand elongation DNA repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion transcription from RNA polymerase II promoter mitochondrion organization transforming growth factor beta receptor signaling pathway protein autoprocessing protein sumoylation signal transduction involved in regulation of gene expression macrophage differentiation mitochondrial DNA metabolic process cellular response to insulin stimulus nucleotide-excision repair, DNA incision cellular response to oxidative stress protein modification process DNA damage response, detection of DNA damage mitochondrial DNA repair positive regulation of transcription from RNA polymerase II promoter DNA ligation involved in DNA repair positive regulation of SMAD protein import into nucleus global genome nucleotide-excision repair regulation of cellular protein localization negative regulation of telomere maintenance via telomere lengthening positive regulation of transcription regulatory region DNA binding telomere maintenance DNA metabolic process base-excision repair transcription, DNA-templated regulation of growth rate cellular response to superoxide regulation of transcription, DNA-templated behavioral response to cocaine regulation of single strand break repair

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PARP1 Monoclonal Antibody (C-2-10)

PARP1 Antibody (MA3-950) in ICC/IF

Product Details

MA3-950

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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