Invitrogen

FAK Monoclonal Antibody (ZF002), Alexa Fluor™ 555

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Datasheet
Protocols
Questions & Answers
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Product Details

MA3-96500-A555

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ZF002

Immunogen

Recombinant protein derived from the N-terminal region of the mouse and rat FAK-1 proteins
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Conjugate

Alexa Fluor™ 555 Alexa Fluor™ 555 Alexa Fluor™ 555

Excitation/Emission Max

553/568 nm View spectra spectra

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS with 1.0mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Dry ice (international)

RRID

AB_2724278

Product Specific Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of FAK, and involved in its initial activation. This phosphorylated site binds Src family SH2 domains and the p85 subunit of PI3-Kinase, and activates cell migration and invasion.

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2

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Gene Aliases: FADK; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2

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UniProt ID: (Human) Q05397, (Mouse) Q5DTH7, (Rat) O35346

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Entrez Gene ID: (Human) 5747, (Mouse) 14083, (Rat) 25614

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Function(s)
actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding ATP binding JUN kinase binding protein kinase binding SH2 domain binding nucleotide binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding identical protein binding phosphatidylinositol 3-kinase binding
Process(es)
MAPK cascade microtubule cytoskeleton organization angiogenesis blood vessel development vasculogenesis neuron migration regulation of protein phosphorylation positive regulation of protein phosphorylation protein phosphorylation movement of cell or subcellular component cell adhesion signal complex assembly epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway JNK cascade multicellular organism development positive regulation of cell proliferation regulation of cell shape regulation of epithelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of smooth muscle cell migration phosphorylation peptidyl-tyrosine phosphorylation central nervous system neuron axonogenesis negative regulation of cell-cell adhesion regulation of cell adhesion extracellular matrix organization positive regulation of cell growth positive regulation of cell migration negative regulation of cell migration cellular chloride ion homeostasis regulation of cell adhesion mediated by integrin peptidyl-tyrosine autophosphorylation establishment of nucleus localization regulation of cell proliferation negative regulation of apoptotic process endothelial cell migration innate immune response regulation of osteoblast differentiation positive regulation of cell adhesion positive regulation of protein kinase activity positive regulation of vasodilation negative regulation of organ growth protein autophosphorylation ephrin receptor signaling pathway positive regulation of smooth muscle cell proliferation positive regulation of phagocytosis negative regulation of axonogenesis positive regulation of synaptic transmission regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of synapse assembly positive regulation of glial cell proliferation growth hormone receptor signaling pathway cellular response to transforming growth factor beta stimulus regulation of substrate adhesion-dependent cell spreading positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of anoikis placenta development heart morphogenesis cellular component disassembly involved in execution phase of apoptosis axon guidance embryo development regulation of endothelial cell migration establishment of cell polarity netrin-activated signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis regulation of GTPase activity positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity vascular endothelial growth factor receptor signaling pathway cell motility regulation of cytoskeleton organization cytoskeleton organization response to mechanical stimulus response to glucose response to organic substance response to organonitrogen compound response to organic cyclic compound response to estradiol response to drug fat cell differentiation response to arsenic-containing substance
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