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Invitrogen
CENPA Monoclonal Antibody (H.577.2)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
CENPA Antibody (MA5-14829) in ICC/IF
Immunofluorescence analysis of CENPA was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CENPA Monoclonal Antibody (H.577.2) (Product # MA5-14829) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # ... View More
Product Details
MA5-14829
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
H.577.2
Immunogen
Synthetic peptide corresponding to the amino terminus of mouse CENP-A protein
Conjugate
Form
Liquid
Concentration
23.4 µg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_10980200
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other histone proteins, including Histone H3.
Target Information
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CENP A; CENP-A; Centromere protein A; Histone H3-like centromeric protein A
Gene Aliases: Cenp-A; Cenpa
UniProt ID: (Mouse) O35216
Entrez Gene ID: (Mouse) 12615
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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