Invitrogen

GAPDH Loading Control Monoclonal Antibody (GA1R), Biotin

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Antibody Testing Data (14)
Published Figures (21)

GAPDH Loading Control Antibody in Western Blot (WB)

FIGURE: 1 / 35

GAPDH Loading Control Antibody (MA5-15738-BTIN) in WB

Western blot analysis of GAPDH was performed by loading 50 µg of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a biotinylated GAPDH monoclonal antibody (Product # MA5-15738-BTIN) at a dilution of 1:1000 for 1 hour at room temperature, followed by Streptavidin-... View More

GAPDH Loading Control Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GAPDH Loading Control Antibody in Immunocytochemistry (ICC/IF)

GAPDH Loading Control Antibody in Flow Cytometry (Flow)

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Product Details

MA5-15738-BTIN

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

Flow Cytometry (Flow)

2 µg/test

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Bacteria, Chicken, Hamster, Human, Insect, Mouse, Rabbit, Rat, Yeast

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GA1R

Immunogen

Recombinant GAPDH

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with proprietary stabilizer

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537653

Product Specific Information

MA5-15738-BTIN has successfully been used for Western blot, FACS, ELISA, IHC (P) and ICC/IF applications.

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Overexpression of HIPK2 removes the transrepression of proapoptotic genes mediated by the CtBP1-p300-FOXO3a complex and increases the chemosensitivity in osteosarcoma cells.

Journal of Cancer

Duan N,Zhang W,Li Z,Sun L,Song T,Yu Z,Chen X,Ma W

Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-BTIN) in Western Blot

Tue Feb 23 00:00:00 EST 2021

Targeting the CtBP1-FOXM1 transcriptional complex with small molecules to overcome MDR1-mediated chemoresistance in osteosarcoma cancer stem cells.

Journal of Cancer

Chen X,Zhang Q,Dang X,Song T,Wang Y,Yu Z,Zhang S,Fan J,Cong F,Zhang W,Duan N

Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-BTIN) in Western Blot

Tue Jan 05 00:00:00 EST 2021

O-GlcNAcylation Enhances NUSAP1 Stability and Promotes Bladder Cancer Aggressiveness.

OncoTargets and therapy

Chen Y,Liu J,Zhang W,Kadier A,Wang R,Zhang H,Yao X

MA5-15738-BTIN was used in Western Blotting to investigate the effects of O-GlcNAcylation in bladder cancer and if it can regulate NUSAP1 protein.

Wed Apr 20 00:00:00 EDT 2022

miR-29a Is Repressed by MYC in Pancreatic Cancer and Its Restoration Drives Tumor-Suppressive Effects via Downregulation of LOXL2.

Molecular cancer research : MCR

Dey S,Kwon JJ,Liu S,Hodge GA,Taleb S,Zimmers TA,Wan J,Kota J

Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-BTIN) in Western Blot

Sat Feb 01 00:00:00 EST 2020

MiR-302b-5p enhances the neuroprotective effect of IGF-1 in methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's disease by regulating inducible nitric-oxide synthase.

Cell biochemistry and function

Cui X,Li M,He Z,Hu L,Liu J,Yan J,Hua L

MA5-15738-BTIN was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to explore that IGF-1 was decreased while iNOS was boosted in MPTP-induced PD mice model.

Tue Dec 01 00:00:00 EST 2020

Specific knockdown of WNT8b expression protects against phosphate-induced calcification in vascular smooth muscle cells by inhibiting the Wnt-β-catenin signaling pathway.

Journal of cellular physiology

Tian BY,Yao L,Sheng ZT,Wan PZ,Qiu XB,Wang J,Xu TH

MA5-15738-BTIN was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate that WNT8b knockdown terminates phosphate-induced VC in VSMCs by inhibiting the Wnt-β-catenin signaling pathway.

Mon Apr 01 00:00:00 EDT 2019

Correction of cognitive deficits in mouse models of Down syndrome by a pharmacological inhibitor of DYRK1A.

Disease models & mechanisms

Nguyen TL,Duchon A,Manousopoulou A,Loaëc N,Villiers B,Pani G,Karatas M,Mechling AE,Harsan LA,Limanton E,Bazureau JP,Carreaux F,Garbis SD,Meijer L,Herault Y

Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-BTIN) in Western Blot

Thu Sep 27 00:00:00 EDT 2018

Estradiol signaling mediates gender difference in visceral adiposity via autophagy.

Cell death & disease

Tao Z,Zheng LD,Smith C,Luo J,Robinson A,Almeida FA,Wang Z,Olumi AF,Liu D,Cheng Z

Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-BTIN) in Western Blot

Thu Feb 22 00:00:00 EST 2018

Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats.

Molecular medicine reports

Wu Q,Dai Q,Jiang L,Wang Y,Yang T,Miao J,Wang J,Han Y

MA5-15738-BTIN was used in Western Blotting to determine the role of microRNA-448 in isoflourane-induced learning and memory impairment in rats.

Tue Aug 01 00:00:00 EDT 2017

Activation of PPARγ by baicalin attenuates pulmonary hypertension in an infant rat model by suppressing HMGB1/RAGE signaling.

FEBS open bio

Chen Z,Wang Q

Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-BTIN) in Western Blot

Sat Apr 01 00:00:00 EDT 2017

Low Frequency Magnetic Fields Induce Autophagy-associated Cell Death in Lung Cancer through miR-486-mediated Inhibition of Akt/mTOR Signaling Pathway.

Scientific reports

Xu Y,Wang Y,Yao A,Xu Z,Dou H,Shen S,Hou Y,Wang T

MA5-15738-BTIN was used in Western Blotting to prove that low frequency magnetic fields can inhibit lung cancers through miR-486 induced autophagic cell death.

Mon Sep 18 00:00:00 EDT 2017

Low Frequency Magnetic Fields Induce Autophagy-associated Cell Death in Lung Cancer through miR-486-mediated Inhibition of Akt/mTOR Signaling Pathway.

Scientific reports

Xu Y,Wang Y,Yao A,Xu Z,Dou H,Shen S,Hou Y,Wang T

MA5-15738-BTIN was used in Western Blotting to prove that low frequency magnetic fields can inhibit lung cancers through miR-486 induced autophagic cell death.

Mon Sep 18 00:00:00 EDT 2017

Degradation of cofilin is regulated by Cbl, AIP4 and Syk resulting in increased migration of LMP2A positive nasopharyngeal carcinoma cells.

Scientific reports

Gainullin MR,Zhukov IY,Zhou X,Mo Y,Astakhova L,Ernberg I,Matskova L

MA5-15738-BTIN was used in Western Blotting to show that under relaxed cellular control, cofilin facilitates tumour cell movement and dissemination.

Mon Aug 21 00:00:00 EDT 2017

Protein-Protein Interactions Suggest Novel Activities of Human Cytomegalovirus Tegument Protein pUL103.

Journal of virology

Ortiz DA,Glassbrook JE,Pellett PE

Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-BTIN) in Western Blot

Thu Sep 01 00:00:00 EDT 2016

The amino-terminal tails of histones H2A and H3 coordinate efficient base excision repair, DNA damage signaling and postreplication repair in Saccharomyces cerevisiae.

Nucleic acids research

Meas R,Smerdon MJ,Wyrick JJ

Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-BTIN) in Western Blot

Tue May 26 00:00:00 EDT 2015

Legionella suppresses the host unfolded protein response via multiple mechanisms.

Nature communications

Treacy-Abarca S,Mukherjee S

Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-BTIN) in Western Blot

Wed Jul 29 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; glceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; multifunctional protein, glycolytic enzyme; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; unnamed protein product

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Gene Aliases: BARS-38; CDABP0047; G3PD; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; I79_001391; KNC-NDS6; OK/SW-cl.12

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UniProt ID: (Rabbit) P46406, (Human) P04406, (Chicken) P00356, (Human) O14556, (Mouse) P16858, (Chinese hamster) P17244, (Rat) P04797, (Rat) Q9ESV6, (Mouse) Q64467

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Entrez Gene ID: (Rabbit) 100009074, (Human) 2597, (Chicken) 374193, (Human) 26330, (Rabbit) 100352213, (Mouse) 14433, (Chinese hamster) 100736557, (Rat) 24383, (Rat) 66020, (Mouse) 14447, (Chinese hamster) 100767862

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Function(s)

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity protein binding microtubule binding peptidyl-cysteine S-nitrosylase activity identical protein binding NADP binding NAD binding aspartic-type endopeptidase inhibitor activity disordered domain specific binding molecular_function oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor dehydrogenase

Process(es)

microtubule cytoskeleton organization gluconeogenesis regulation of macroautophagy negative regulation of translation peptidyl-cysteine S-trans-nitrosylation protein stabilization neuron apoptotic process canonical glycolysis cellular response to interferon-gamma nuclear membrane fusion glycolytic process negative regulation of endopeptidase activity negative regulation of apoptotic process positive regulation of cytokine secretion defense response to fungus killing by host of symbiont cells positive regulation by organism of apoptotic process in other organism involved in symbiotic interaction oxidation-reduction process antimicrobial humoral immune response mediated by antimicrobial peptide spermatid development sperm motility positive regulation of glycolytic process carbohydrate metabolic process apoptotic process multicellular organism development response to ammonium ion regulation of translation glucose metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GAPDH Loading Control Monoclonal Antibody (GA1R), Biotin

GAPDH Loading Control Antibody (MA5-15738-BTIN) in WB

Product Details

MA5-15738-BTIN

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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