Invitrogen

TLR4 Monoclonal Antibody (HTA125), FITC

View all (67) TLR4 (CD284) antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-16208

Applications
Tested Dilution
Publications

Immunohistochemistry (IHC)

Assay-Dependent
-

Immunocytochemistry (ICC/IF)

Assay-Dependent
-

Flow Cytometry (Flow)

1 µL/ 1 million cells
-
Product Specifications

Species Reactivity

Dog, Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

HTA125

Immunogen

Ba/F3 cell line expressing human TLR4 cell surface antigen
View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

50mM sodium borate

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537726

Product Specific Information

This antibody has been demonstrated to block activation of monocytes with LPS.

Target Information

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD284; homolog of Drosophila toll; hToll; lipopolysaccharide response; Toll-like receptor 4; Toll-like receptor4 protein

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Gene Aliases: ARMD10; CD284; Lps; Ly87; Ran/M1; Rasl2-8; TLR-4; TLR4; TOLL

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UniProt ID: (Human) O00206, (Mouse) Q9QUK6

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Entrez Gene ID: (Human) 7099, (Dog) 403417, (Mouse) 21898

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Function(s)
lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding phosphatidylinositol 3-kinase binding
Process(es)
activation of MAPK activity microglial cell activation activation of innate immune response toll-like receptor signaling pathway B cell proliferation involved in immune response immune system process nitric oxide production involved in inflammatory response regulation of dendritic cell cytokine production MyD88-dependent toll-like receptor signaling pathway innate immune response-activating signal transduction leukotriene metabolic process inflammatory response response to oxidative stress signal transduction activation of NF-kappaB-inducing kinase activity I-kappaB phosphorylation response to bacterium positive regulation of platelet activation positive regulation of B cell proliferation lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide detection of lipopolysaccharide interferon-gamma production negative regulation of interferon-gamma production negative regulation of interleukin-17 production negative regulation of interleukin-23 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of chemokine production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interferon-gamma production positive regulation of interleukin-1 production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of stress-activated MAPK cascade toll-like receptor 4 signaling pathway macrophage activation positive regulation of NF-kappaB import into nucleus positive regulation of tumor necrosis factor biosynthetic process positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of DNA binding positive regulation of interleukin-12 biosynthetic process innate immune response positive regulation of MHC class II biosynthetic process positive regulation of interferon-beta biosynthetic process positive regulation of interleukin-1 biosynthetic process positive regulation of interleukin-13 biosynthetic process positive regulation of interleukin-6 biosynthetic process positive regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process response to ethanol mast cell activation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of lymphocyte proliferation regulation of cytokine secretion regulation of inflammatory response positive regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of nitric-oxide synthase biosynthetic process regulation of sensory perception of pain intestinal epithelial structure maintenance positive regulation of macrophage cytokine production negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway response to fatty acid cellular response to lipopolysaccharide cellular response to lipoteichoic acid MyD88-independent toll-like receptor signaling pathway immune response I-kappaB kinase/NF-kappaB signaling positive regulation of gene expression astrocyte development detection of fungus negative regulation of MyD88-independent toll-like receptor signaling pathway TRIF-dependent toll-like receptor signaling pathway T-helper 1 type immune response defense response to bacterium negative regulation of osteoclast differentiation interleukin-1 beta secretion necroptotic process cellular response to mechanical stimulus apoptotic signaling pathway positive regulation of NLRP3 inflammasome complex assembly
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