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TLR5 Antibody (MA5-16237) in IHC (P)
Immunohistochemical analysis of TLR5 in formalin-fixed paraffin-embedded tissue section of normal human skin. Samples were incubated in TLR5 monoclonal antibody (Product # MA5-16237) using a dilution of 5 µg/mL. Intense membrane staining of TLR5 was observed specifically in the pigmented basel cells of epidermal layer [10X Magnification].
Product Details
MA5-16237
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
19D759.2
Immunogen
KLH-conjugated synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2537755
Target Information
TLR5 is a Type I membrane protein belonging to the Toll-like receptor family, participates in the innate immune response to microbial agents. TLR5 also plays a role in mediating detection of bacterial flagellins. TLR5 acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. TLR5 protein binds to TIRAP and MyD88 via their respective TIR domains TLR5 is highly expressed in liver, and is detected in lung and at very low levels in most other tissues. The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA). The strain MOLF/Ei is highly susceptible to the infection, has a unique TLR5 haplotype and a lower expression of TRL5. A common TLR5 stop codon polymorphism that disrupts TLR5 signaling is associated with susceptibility to Legionnaires’ disease and demonstrates the importance of TLR5 in the innate immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ10052; MGC126430; MGC126431; RP11-239E10.1; systemic lupus erythematosus susceptibility 1; Toll-like receptor 5; Toll/interleukin-1 receptor-like protein 3
Gene Aliases: MELIOS; SLE1; SLEB1; TIL3; TLR5
UniProt ID: (Human) O60602, (Mouse) Q9JLF7
Entrez Gene ID: (Dog) 488605, (Human) 7100, (Mouse) 53791
positive regulation of cytokine production
immune system process
MyD88-dependent toll-like receptor signaling pathway
inflammatory response
signal transduction
positive regulation of interleukin-8 production
positive regulation of toll-like receptor signaling pathway
toll-like receptor 5 signaling pathway
defense response to bacterium
innate immune response
positive regulation of nitric oxide biosynthetic process
regulation of cytokine secretion
male gonad development
defense response to Gram-negative bacterium
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
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