Invitrogen

CD34 Monoclonal Antibody (QBEND/10), FITC

Product Details

MA5-16925

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 21 publications 21 publications

Flow Cytometry (Flow)

Neat-1:10

Product Specifications

Species Reactivity

Cynomolgus monkey, Human

Published species

Human, Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

QBEND/10

Immunogen

Human endothelial cell membrane vesicles

View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

purified

Storage buffer

PBS with 1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538402

Product Specific Information

This antibody does not react with bovine, dog, rat or sheep.|For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.|Mouse anti Human CD34 antibody, clone QBEND/10 recognizes the human CD34 antigen, also known as Hematopoietic progenitor cell antigen CD34.|Quantity: 100 Tests/1 mL

Target Information

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Rhabdoid morphology in gastrointestinal stromal tumours (GISTs) is associated with PDGFRA mutations but does not imply aggressive behaviour.

Histopathology

Schaefer IM,Ströbel P,Cameron S,Beham A,Otto C,Schildhaus HU,Agaimy A

MA5-16925 was used in immunohistochemistry to study the association of mutations in PDGF-Ralpha with gastrointestinal stromal tumors of rhabdoid morphology

Sat Feb 01 00:00:00 EST 2014

Recruitment of CD34(+) fibroblasts in tumor-associated reactive stroma: the reactive microvasculature hypothesis.

The American journal of pathology

San Martin R,Barron DA,Tuxhorn JA,Ressler SJ,Hayward SW,Shen X,Laucirica R,Wheeler TM,Gutierrez C,Ayala GE,Ittmann M,Rowley DR

MA5-16925 was used in immunohistochemistry to study the recruitment to tumor-associated reactive stroma of a novel CD34(+)/vimentin(+) dual-positive fibroblast

Sun Jun 01 00:00:00 EDT 2014

Use of platelet-rich plasma solution applied with composite chondrocutaneous graft technique: an experimental study in rabbit model.

Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons

Sevim KZ,Yazar M,Irmak F,Tekkeşin MS,Yildiz K,Sirvan SS

MA5-16925 was used in immunohistochemistry to study the mechanisms underlying the beneficial effects of using platelet-rich plasma in composite chondrocutaneous graft procedures

Tue Jul 01 00:00:00 EDT 2014

Superficial soft tissue biphasic synovial sarcoma with apocrine differentiation in the glandular component: a report of two cases.

The American Journal of dermatopathology

Shelekhova KV,Calonje E,Grossmann P,Kacerovska D,Koudela K,Mirka H,Michal M,Kazakov DV

MA5-16925 was used in immunohistochemistry to report on two cases of superficial soft tissue biphasic synovial sarcoma presenting with apocrine differentiation

Wed Oct 01 00:00:00 EDT 2014

Immunohistochemical study of Ki67, CD34 and p53 expression in human tooth buds.

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie

Muica Nagy-Bota MC,Pap Z,Denes L,Ghizdavăţ A,Brînzaniuc K,Lup Coşarcă AS,Chibelean Cireş-Mărginean M,Păcurar M,Pávai Z

MA5-16925 was used in immunohistochemistry to study the developmental time course of Ki67, CD34 and p53 expression in human tooth buds

Mon Apr 06 00:00:00 EDT 2015

Evaluation of protein biomarkers of prostate cancer aggressiveness.

BMC cancer

Rizzardi AE,Rosener NK,Koopmeiners JS,Isaksson Vogel R,Metzger GJ,Forster CL,Marston LO,Tiffany JR,McCarthy JB,Turley EA,Warlick CA,Henriksen JC,Schmechel SC

MA5-16925 was used in immunohistochemistry to screen a panel of 33 proteins to establish a set of biomarkers that predict the agressiveness of prostate cancer

Sat Apr 05 00:00:00 EDT 2014

Molecular analysis of the KIT gene in gastrointestinal stromal tumors with novel mutations.

Applied immunohistochemistry & molecular morphology : AIMM

Calibasi G,Baskin Y,Alyuruk H,Cavas L,Oztop I,Sagol O,Atila K,Ellidokuz H,Yilmaz U

MA5-16925 was used in immunohistochemistry to study the clinicopathology of gastrointestinal stromal tumors with novel KIT mutations

Wed Jan 01 00:00:00 EST 2014

Primary breast cancer stem-like cells metastasise to bone, switch phenotype and acquire a bone tropism signature.

British journal of cancer

D'Amico L,Patanè S,Grange C,Bussolati B,Isella C,Fontani L,Godio L,Cilli M,D'Amelio P,Isaia G,Medico E,Ferracini R,Roato I

MA5-16925 was used in immunohistochemistry to study the bone metastasis of primary breast cancer stem-like cells and the bone-tropism signature displayed by cells isolated from bone

Tue Jun 25 00:00:00 EDT 2013

Incidental Epstein-Barr virus associated atypical lymphoid proliferation arising in a left atrial myxoma: a case of long survival without any postsurgical treatment and review of the literature.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology

Bartoloni G,Pucci A,Giorlandino A,Berretta M,Mignosa C,Italia F,Carbone A,Canzonieri V

MA5-16925 was used in immunohistochemistry to report on a case of left atrial cardiac myxoma with incidental atypical B-cell lymphoid proliferation

Thu Dec 12 00:00:00 EST 2013

Contribution of DOG1 expression to the diagnosis of gastrointestinal stromal tumors.

Pathology, research and practice

Kara T,Serinsoz E,Arpaci RB,Gubur O,Orekici G,Ata A,Colak T,Arican A

MA5-16925 was used in immunohistochemistry to study the diagnostic value of the immunohistochemical expression of DOG1 in gastrointestinal stromal tumors

Mon Jul 01 00:00:00 EDT 2013

Histopathological, immunophenotypic and clinical particularities and evolution of a case of hepatosplenic T-cell lymphoma in transformation to leukemia.

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie

Benedek Lázár E,Köpeczi JB,Tunyogi AB,Kakucs E,Horváth E,Turcu M,Benedek I

MA5-16925 was used in immunohistochemistry to report on a case of hepatosplenic T-cell lymphoma with leukemic transformation

Wed Jul 02 00:00:00 EDT 2014

A rare malignant tumor of scalp in a 3-month-old Taiwanese infancy: case report of primitive myxoid mesenchymal tumor of infancy with molecular study.

Medical molecular morphology

Su TC,Hwang MJ,Li CF,Wang SC,Lee CH,Chen CJ

MA5-16925 was used in immunohistochemistry to report on a case of primitive myxoid mesenchymal tumor of infancy

Sat Jun 01 00:00:00 EDT 2013

The decrease of mineralcorticoid receptor drives angiogenic pathways in colorectal cancer.

PloS one

Tiberio L,Nascimbeni R,Villanacci V,Casella C,Fra A,Vezzoli V,Furlan L,Meyer G,Parrinello G,Baroni MD,Salerni B,Schiaffonati L

MA5-16925 was used in immunohistochemistry to study the regulation of angiogenesis in colorectal cancer by the mineralocorticoid receptor

Tue Sep 24 00:00:00 EDT 2013

The prognostic impact of MGMT expression on low-grade gangliogliomas: a clinicopathological and immunohistochemical study.

Folia neuropathologica

Chang IW,Hsu CT,Lin JW,Hung CH

MA5-16925 was used in immunohistochemistry to study the prognostic value of the immunohistochemical expression of MGMT in low-grade ganglioglioma

Mon Jul 14 00:00:00 EDT 2014

Castration resistant prostate cancer is associated with increased blood vessel stabilization and elevated levels of VEGF and Ang-2.

The Prostate

Tomić TT,Gustavsson H,Wang W,Jennbacken K,Welén K,Damber JE

MA5-16925 was used in immunohistochemistry to study blood vessel stabilization and the expression of pro-angiogenic VEGF and Ang-2 in castration-resistant prostate cancer

Tue May 15 00:00:00 EDT 2012

Stromal expression of CD34, α-smooth muscle actin and CD26/DPPIV in squamous cell carcinoma of the skin: a comparative immunohistochemical study.

Pathology oncology research : POR

Kacar A,Arikok AT,Kokenek Unal TD,Onder E,Hucumenoglu S,Alper M

MA5-16925 was used in immunohistochemistry to compare the immunohistochemical expression of CD34, alpha-smooth muscle actin and CD26 in normal stroma and tumor stroma in squamous cell carcinoma of the skin

Sun Jan 01 00:00:00 EST 2012

Vaginal superficial myofibroblastoma: a rare mesenchymal tumor of the lower female genital tract and a study of its association with viral infection.

Medical molecular morphology

Liu JL,Su TC,Shen KH,Lin SH,Wang HK,Hsu JC,Chen CJ

MA5-16925 was used in immunohistochemistry to report on a case of vaginal superficial myofibroblastoma

Fri Jun 01 00:00:00 EDT 2012

Early onset of endothelial cell proliferation in coronary thrombi of patients with an acute myocardial infarction: implications for plaque healing.

Journal of thrombosis and haemostasis : JTH

Li X,Kramer MC,VAN DER Loos CM,Ploegmakers HJ,DE Boer OJ,Koch KT,Tijssen JG,DE Winter RJ,VAN DER Wal AC

MA5-16925 was used in immunohistochemistry to study neovascularization and the timing of endothelial cell proliferation in coronary thrombi following acute myocardial infarction

Thu Mar 01 00:00:00 EST 2012

Aberrant expression of CD133 in non-small cell lung cancer and its relationship to vasculogenic mimicry.

BMC cancer

Wu S,Yu L,Wang D,Zhou L,Cheng Z,Chai D,Ma L,Tao Y

MA5-16925 was used in immunohistochemistry to study the prognostic value of CD133 and vasculogenic mimicry in patients with non-small cell lung cancer

Wed Nov 21 00:00:00 EST 2012

Expression of maspin in non-small cell lung cancer and its relationship to vasculogenic mimicry.

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban

Wu S,Yu L,Cheng Z,Song W,Zhou L,Tao Y

MA5-16925 was used in immunohistochemistry to study the significance of maspin expression in non-small cell lung cancer

Fri Jun 01 00:00:00 EDT 2012

Human peripheral lymphoid tissues contain autoimmune regulator-expressing dendritic cells.

The American journal of pathology

Poliani PL,Kisand K,Marrella V,Ravanini M,Notarangelo LD,Villa A,Peterson P,Facchetti F

MA5-16925 was used in immunohistochemistry to study the presence of dendritic cells expressing autoimmune regulator in peripheral lymphoid tissue in humans

Mon Mar 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CD34; CD34 antigen; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2; unnamed protein product

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Gene Aliases: CD34

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UniProt ID: (Human) P28906

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Entrez Gene ID: (Human) 947, (Cynomolgus monkey) 101866926

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Function(s)

transcription factor binding carbohydrate binding sulfate binding cell adhesion molecule

Process(es)

tissue homeostasis endothelial cell proliferation glomerular filtration endothelium development cell-matrix adhesion signal transduction regulation of blood pressure cell proliferation positive regulation of gene expression negative regulation of gene expression single organismal cell-cell adhesion hemopoiesis negative regulation of blood coagulation negative regulation of tumor necrosis factor production positive regulation of interleukin-10 production mesangial cell-matrix adhesion paracrine signaling positive regulation of odontogenesis negative regulation of nitric oxide biosynthetic process positive regulation of angiogenesis cell motility regulation of immune response leukocyte migration transdifferentiation vascular wound healing hematopoietic stem cell proliferation positive regulation of transforming growth factor beta production positive regulation of granulocyte colony-stimulating factor production extracellular exosome assembly glomerular endothelium development stem cell proliferation metanephric glomerular mesangial cell differentiation negative regulation of cellular response to heat negative regulation of cellular response to hypoxia negative regulation of interleukin-2 secretion positive regulation of glial cell-derived neurotrophic factor secretion negative regulation of neuron death positive regulation of vasculogenesis

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CD34 Monoclonal Antibody (QBEND/10), FITC

Product Details

MA5-16925

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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