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Product Details
MA5-17837
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
ACK4
Immunogen
IL-3 dependent mast cells derived from WB- +/+ mice
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein G
Storage buffer
PBS with 4-5mg/mL BSA
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539221
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: belly-spot; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit
Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; Kit; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W
UniProt ID: (Mouse) P05532
Entrez Gene ID: (Mouse) 16590
nucleotide binding
protease binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
stem cell factor receptor activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
cytokine binding
protein homodimerization activity
metal ion binding
transmembrane signal receptor
activation of MAPK activity
ovarian follicle development
myeloid progenitor cell differentiation
lymphoid progenitor cell differentiation
immature B cell differentiation
dendritic cell cytokine production
mast cell chemotaxis
myeloid leukocyte differentiation
protein phosphorylation
glycosphingolipid metabolic process
chemotaxis
inflammatory response
transmembrane receptor protein tyrosine kinase signaling pathway
germ cell development
spermatogenesis
spermatid development
positive regulation of cell proliferation
germ cell migration
regulation of cell shape
visual learning
response to radiation
positive regulation of gene expression
phosphorylation
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
lamellipodium assembly
hemopoiesis
cell differentiation
T cell differentiation
erythrocyte differentiation
melanocyte differentiation
positive regulation of cell migration
positive regulation of pseudopodium assembly
actin cytoskeleton reorganization
mast cell cytokine production
somatic stem cell population maintenance
embryonic hemopoiesis
ectopic germ cell programmed cell death
intracellular signal transduction
megakaryocyte development
Fc receptor signaling pathway
Kit signaling pathway
erythropoietin-mediated signaling pathway
positive regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of programmed cell death
mast cell degranulation
positive regulation of MAP kinase activity
positive regulation of MAPK cascade
pigmentation
positive regulation of Notch signaling pathway
positive regulation of JAK-STAT cascade
protein autophosphorylation
developmental pigmentation
regulation of developmental pigmentation
somatic stem cell division
positive regulation of long-term neuronal synaptic plasticity
digestive tract development
stem cell differentiation
epithelial cell proliferation
detection of mechanical stimulus involved in sensory perception of sound
positive regulation of sequence-specific DNA binding transcription factor activity
cell chemotaxis
mast cell differentiation
cellular response to thyroid hormone stimulus
melanocyte migration
melanocyte adhesion
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