Invitrogen

CD8 beta Monoclonal Antibody (CT-CD8b)

CD8 beta Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

CD8 beta Antibody (MA5-17843) in Flow

Flow cytometric analysis of Thymus cells stained using anti-CD8b/LYT3 Monoclonal Antibody (Product # MA5-17843) at a 0.1 µg/10^6 cells dilution.

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Product Details

MA5-17843

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.1 µg/1x10^6 cells

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 10 publications 10 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

CT-CD8b

Immunogen

Mouse CD8 β

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539227

Product Specific Information

MA5-17843 targets CD8b/LYT3 in FACS applications and shows reactivity with Mouse samples.

The MA5-17843 immunogen is mouse CD8 ß.

Target Information

The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Maternal immune stimulation during pregnancy shapes the immunological phenotype of offspring.

Brain, behavior, and immunity

Mandal M,Donnelly R,Elkabes S,Zhang P,Davini D,David BT,Ponzio NM

MA5-17843 was used in flow cytometry to use poly(I:C)-injected dams to study the effects of inflammation on fetal development.

Tue Oct 01 00:00:00 EDT 2013

Inactivation of tumor-specific CD8⁺ CTLs by tumor-infiltrating tolerogenic dendritic cells.

Immunology and cell biology

Harimoto H,Shimizu M,Nakagawa Y,Nakatsuka K,Wakabayashi A,Sakamoto C,Takahashi H

MA5-17843 was used in flow cytometry to elucidate the role of dendritic cells in cancer immunosurveillance failure

Tue Oct 01 00:00:00 EDT 2013

Anti-CD8 antibodies can trigger CD8+ T cell effector function in the absence of TCR engagement and improve peptide-MHCI tetramer staining.

Journal of immunology (Baltimore, Md. : 1950)

Clement M,Ladell K,Ekeruche-Makinde J,Miles JJ,Edwards ES,Dolton G,Williams T,Schauenburg AJ,Cole DK,Lauder SN,Gallimore AM,Godkin AJ,Burrows SR,Price DA,Sewell AK,Wooldridge L

MA5-17843 was used in flow cytometry to test if anti-CD8 Abs induce effector function.

Fri Jul 15 00:00:00 EDT 2011

Thymic alterations in mice deficient for the SNARE protein VAMP8/endobrevin.

Cell and tissue research

Kanwar N,Fayyazi A,Backofen B,Nitsche M,Dressel R,von Mollard GF

MA5-17843 was used in flow cytometry to elucidate the role of VAMP8 in the thymus

Sat Nov 01 00:00:00 EDT 2008

Defective proximal TCR signaling inhibits CD8+ tumor-infiltrating lymphocyte lytic function.

Journal of immunology (Baltimore, Md. : 1950)

Koneru M,Schaer D,Monu N,Ayala A,Frey AB

MA5-17843 was used to elucidate the lack of cytolysis of CD8+ tumor-infiltrating lymphocytes

Tue Feb 15 00:00:00 EST 2005

The point mutation of tyrosine 759 of the IL-6 family cytokine receptor gp130 synergizes with HTLV-1 pX in promoting rheumatoid arthritis-like arthritis.

International immunology

Ishihara K,Sawa S,Ikushima H,Hirota S,Atsumi T,Kamimura D,Park SJ,Murakami M,Kitamura Y,Iwakura Y,Hirano T

MA5-17843 was used in flow cytometry to characterize a new mouse model of rheumatoid arthritis.

Mon Mar 01 00:00:00 EST 2004

High avidity antigen-specific CTL identified by CD8-independent tetramer staining.

Journal of immunology (Baltimore, Md. : 1950)

Choi EM,Chen JL,Wooldridge L,Salio M,Lissina A,Lissin N,Hermans IF,Silk JD,Mirza F,Palmowski MJ,Dunbar PR,Jakobsen BK,Sewell AK,Cerundolo V

MA5-17843 was used in flow cytometry to test if tetramers distinguish between high and low avidity T cell clones.

Sat Nov 15 00:00:00 EST 2003

CD8+ tumor-infiltrating lymphocytes are primed for Fas-mediated activation-induced cell death but are not apoptotic in situ.

Journal of immunology (Baltimore, Md. : 1950)

Radoja S,Saio M,Frey AB

MA5-17843 was used in flow cytometry to characterize murine tumor-infiltrating lymphocytes.

Tue May 15 00:00:00 EDT 2001

Endogenous inhibition of antimycobacterial immunity by IL-10 varies between mycobacterial species.

Scandinavian journal of immunology

Roach DR,Martin E,Bean AG,Rennick DM,Briscoe H,Britton WJ

MA5-17843 was used in flow cytometry to elucidate the role of IL-10 in mycobacterial infections.

Thu Aug 09 00:00:00 EDT 2001

Characterization of peripheral regulatory CD4+ T cells that prevent diabetes onset in nonobese diabetic mice.

Journal of immunology (Baltimore, Md. : 1950)

Lepault F,Gagnerault MC

MA5-17843 was used in flow cytometry to determine the phenotype and function of T cells that prevent diabetes.

Sat Jan 01 00:00:00 EST 2000

Lymphoid hyperplasia, autoimmunity, and compromised intestinal intraepithelial lymphocyte development in colitis-free gnotobiotic IL-2-deficient mice.

Journal of immunology (Baltimore, Md. : 1950)

Contractor NV,Bassiri H,Reya T,Park AY,Baumgart DC,Wasik MA,Emerson SG,Carding SR

MA5-17843 was used in flow cytometry to determine if the microbiota contributes to autoimmunity in IL-2 deficient mice.

Thu Jan 01 00:00:00 EST 1998

Targets of p56(lck) activity in immature thymoblasts: stimulation of the Ras/Raf/MAPK pathway.

International immunology

Lin K,Abraham KM

MA5-17843 was used in flow cytometry to demonstrate that the Ras/Raf/MAPK pathway is constitutively activated in lck-transformed immature thymoblasts.

Sat Feb 01 00:00:00 EST 1997

Bioinformatics

Protein Aliases: CD8; CD8alpha; CD8b; CD8beta; Leu-2; Lymphocyte antigen 3; MAL; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-3

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Gene Aliases: Cd8b; Cd8b1; Ly-3; Ly-C; Lyt-3; Lyt3

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UniProt ID: (Mouse) P10300

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Entrez Gene ID: (Mouse) 12526

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Function(s)

protein binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

immune system process

Performance Guarantee

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CD8 beta Monoclonal Antibody (CT-CD8b)

CD8 beta Antibody (MA5-17843) in Flow

Product Details

MA5-17843

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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