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LCK Antibody (MA5-18166) in Flow
Separation of human LCK positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human LCK (LCK-01) Alexa Fluor® 647 Monoclonal antibody (Product # MA5-18166), concentration in sample 8 µg/mL.
Product Details
MA5-18166
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
LCK-01
Immunogen
Peptide corresponding to amino acids 22-36 in the sequence of human Lck
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS
Contains
15mM sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539540
Product Specific Information
This antibody recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase (intracellular antigen). This antibody will not cross-react with mouse.
Target Information
LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 2.7.10.2; kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; p56(LSTRA) protein-tyrosine kinase; p56-LCK; Protein YT16; Proto-oncogene Lck; proto-oncogene tyrosine-protein kinase LCK; RP4-675E8.4; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyr; Tyrosine-protein kinase Lck
Gene Aliases: IMD22; LCK; LSK; p56lck; pp58lck; YT16
UniProt ID: (Human) P06239
Entrez Gene ID: (Human) 3932
glycoprotein binding
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein serine/threonine phosphatase activity
protein binding
ATP binding
protein C-terminus binding
kinase activity
protein kinase binding
protein phosphatase binding
SH2 domain binding
CD4 receptor binding
CD8 receptor binding
identical protein binding
phosphatidylinositol 3-kinase binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
ATPase binding
protein phosphorylation
protein dephosphorylation
cellular zinc ion homeostasis
activation of cysteine-type endopeptidase activity involved in apoptotic process
transmembrane receptor protein tyrosine kinase signaling pathway
regulation of phosphatidylinositol 3-kinase signaling
viral process
hemopoiesis
platelet activation
T cell differentiation
T cell costimulation
peptidyl-tyrosine autophosphorylation
regulation of cell proliferation
response to drug
innate immune response
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
regulation of defense response to virus by virus
T cell receptor signaling pathway
B cell receptor signaling pathway
positive regulation of T cell receptor signaling pathway
positive regulation of T cell activation
leukocyte migration
release of sequestered calcium ion into cytosol
regulation of lymphocyte activation
positive regulation of intrinsic apoptotic signaling pathway
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