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Invitrogen
VAChT Monoclonal Antibody (N6/38)
FIGURE: 1 / 3
VAChT Antibody (MA5-27662) in ICC/IF
Immunofluorescent analysis of VAChT in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with VAChT monoclonal antibody (Product # MA5-27662) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) VAChT Antibody, D) Merged image. Magnification... View More
Product Details
MA5-27662
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N6/38
Immunogen
Synthetic peptide amino acids 521-532 of human VAChT
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2735407
Product Specific Information
A dilution of 1:50-1:200 of MA5-27662 was sufficient for detection of VAChT Transporter in rat brain using immunohistochemistry analysis and goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 56kDa.
This antibody was formerly sold as S6-38.
Target Information
This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MGC12716; solute carrier family 18 (vesicular acetylcholine transporter), member 3; solute carrier family 18 (vesicular acetylcholine), member 3; solute carrier family 18 (vesicular monoamine) member 3; Solute carrier family 18 member 3; solute carrier family 18, member 3; VAChT; Vesicular acetylcholine transporter
Gene Aliases: rVAT; SLC18A3; VACHT; VAT
UniProt ID: (Human) B2R7S1, (Rat) Q62666
Entrez Gene ID: (Human) 6572, (Mouse) 20508, (Rat) 60422
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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