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FGF13 Antibody (MA5-27705) in ICC/IF
Immunofluorescent analysis of FGF13 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with FGF13 monoclonal antibody (Product # MA5-27705) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) FGFA/FHFA (pan) Antibody, D) Merged image. Mag... View More
Product Details
MA5-27705
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
N235/22
Immunogen
Synthetic peptide amino acids 2-18 (AAAIASSLIRQKRQARE) of human FHF2A. 100% identical to rat, 94% identical to mouse. >80% identity with FGF12A/FHF1A, FGF14A/FHF4A and FGF11A/FHF3A.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2735180
Product Specific Information
1 µg/mL of MA5-27705 was sufficient for detection of FGFA/FHFA (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 30kDa. Does not cross-react with FGF13B/FHF2B. Cross reacts with FGF12A/FHF1A and FGF14A/FHF4A.
This antibody was formerly sold as clone S235-22.
Target Information
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This gene is located in a region on chromosome X, which is associated with Borjeson-Forssman-Lehmann syndrome (BFLS), making it a possible candidate gene for familial cases of the BFLS, and for other syndromal and nonspecific forms of X-linked mental retardation mapping to this region. Alternative splicing of this gene at the 5' end results in several transcript variants encoding different isoforms with different N-termini.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AFGF; ECGFA; ECGFB; FGF; FGF 1; FGF alpha; FGF-13; FGF13A; FGFA; FHF-2; Fibroblast growth factor; Fibroblast growth factor 13; Fibroblast growth factor homologous factor 2; GLIO703; HBGF 1; HBGF1
Gene Aliases: FGF-13; FGF13; FGF2; FHF-2; FHF2
UniProt ID: (Human) Q92913, (Rat) Q9ERW3, (Mouse) P70377
Entrez Gene ID: (Human) 2258, (Rat) 84488, (Mouse) 14168
MAPK cascade
neuron migration
negative regulation of microtubule depolymerization
signal transduction
cell-cell signaling
nervous system development
learning
memory
hippocampus development
cerebral cortex cell migration
activation of protein kinase activity
establishment of neuroblast polarity
microtubule polymerization
negative regulation of collateral sprouting
protein localization to plasma membrane
apoptotic process
cell proliferation
positive regulation of protein kinase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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