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CENPA Antibody (MA5-27710) in ICC/IF
Immunofluorescent analysis of CENPA in human colon cancer cell line (HT-29). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with CENPA monoclonal antibody (Product # MA5-27710) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100 (60 min at RT) and 1:5000 (5 min at RT). Magnification: 60x.
Product Details
MA5-27710
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5A7-2E11
Immunogen
Synthetic peptide corresponding to a portion of human CENP-A (NH2-PRRRSRKPEAPRRRSPS-COOH).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2735143
Product Specific Information
1 µg/mL of MA5-27710 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 18kDa. Recognizes Human CENP-A.
Target Information
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CENP A; CENP-A; Centromere autoantigen A; Centromere protein A; centromere protein A, 17kDa; centromere-specific histone; Histone H3-like centromeric protein A
Gene Aliases: CenH3; CENP-A; CENPA
UniProt ID: (Human) P49450
Entrez Gene ID: (Human) 1058
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