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Invitrogen
15-PGDH Recombinant Rabbit Monoclonal Antibody (016)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
15-PGDH Antibody (MA5-29327) in ICC/IF
Immunofluorescence staining of Human 15-PGDH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with 15-PGDH Recombinant Rabbit Monoclonal Antibody (16) (Product # MA5-29327, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Product Details
MA5-29327
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
016
Immunogen
Recombinant Human HPGD/15-PGDH protein (Met1-Gln266)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785213
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human HPGD/15-PGDH.
Target Information
Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 15-hydroxyprostaglandin dehydrogenase [NAD(+)]; 15-PGDH; Eicosanoid/docosanoid dehydrogenase [NAD(+)]; NAD+-dependent 15-hydroxyprostaglandin dehydrogenase; Prostaglandin dehydrogenase 1; Short chain dehydrogenase/reductase family 36C member 1; short chain dehydrogenase/reductase family 36C, member 1
Gene Aliases: 15-PGDH; HPGD; PGDH; PGDH1; PHOAR1; SDR36C1
UniProt ID: (Human) P15428
Entrez Gene ID: (Human) 3248
prostaglandin metabolic process
transforming growth factor beta receptor signaling pathway
female pregnancy
parturition
lipoxygenase pathway
ovulation
negative regulation of cell cycle
oxidation-reduction process
thrombin receptor signaling pathway
ductus arteriosus closure
lipoxin metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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