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TROP2 Antibody (MA5-29593) in ICC/IF
Immunofluorescence analysis of TROP2 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TROP2 Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29593) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus ... View More
Product Details
MA5-29593
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
001
Immunogen
Recombinant Human TROP2/TACSTD2 protein (Met1-Thr274)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785434
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human TROP2/TACSTD2.
Target Information
TROP2 is a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of its gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 40kD glycoprotein, identified by monoclonal antibody GA733; Cell surface glycoprotein Trop-2; cell surface glycoprotein TROP2; epithelial glycoprotein-1; GA733; gastrointestinal tumor-associated antigen GA7331; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331; TACD2; truncated TACSTD2; Tumor-associated calcium signal transducer 2
Gene Aliases: EGP-1; EGP1; GA733-1; GA7331; GP50; M1S1; TACSTD2; TROP2
UniProt ID: (Human) P09758
Entrez Gene ID: (Human) 4070
cell surface receptor signaling pathway
visual perception
cell proliferation
negative regulation of epithelial cell migration
regulation of epithelial cell proliferation
negative regulation of stress fiber assembly
ureteric bud morphogenesis
negative regulation of branching involved in ureteric bud morphogenesis
cell-cell adhesion
negative regulation of substrate adhesion-dependent cell spreading
negative regulation of ruffle assembly
negative regulation of cell motility
positive regulation of stem cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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