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IL4R Antibody (MA5-31309) in Flow
Flow cytometric analysis of rat IL4R expression on SD rat splenocytes. Cells were stained with IL4R Recombinant Rabbit Monoclonal Antibody (2) (Product # MA5-31309), then a FITC-conjugated Secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Product Details
MA5-31309
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
002
Immunogen
Recombinant Rat IL-4R/CD124 Protein (Met1-Arg232)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2786946
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Rat IL-4R/CD124.
Target Information
IL4 Receptor alpha (IL4RA) is the alpha chain of the interleukin 4 receptor which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine and mucus production at sites of allergic inflammmation. The IL4 response is involved in promoting Th2 differentiation. The secreted extracellular domain of IL4R alpha, called sIL4R alpha, can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. It has no signaling abilities. Allelic variations in the IL4RA gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD124; il-4 receptor; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; interleukin 4 receptor, alpha; interleukin-4 receptor alpha chain; Interleukin-4 receptor subunit alpha
Gene Aliases: Il4r; Il4ra
UniProt ID: (Rat) Q63257
Entrez Gene ID: (Rat) 25084
production of molecular mediator involved in inflammatory response
positive regulation of immunoglobulin production
inflammatory response
immune response
immunoglobulin mediated immune response
ovulation
interleukin-4-mediated signaling pathway
regulation of cell proliferation
defense response to protozoan
positive regulation of macrophage activation
positive regulation of mast cell degranulation
response to estrogen
negative regulation of T-helper 1 cell differentiation
positive regulation of T-helper 2 cell differentiation
positive regulation of chemokine secretion
positive regulation of myoblast fusion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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