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Invitrogen
NEFM Recombinant Rabbit Monoclonal Antibody (JM11-20)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 11
NEFM Antibody (MA5-32613) in ICC/IF
Knockout of NEFM was achieved by CRISPR-Cas9 genome editing. Immunofluorescence analysis was performed on wild type HEK-293 cells (panel a,d), HEK-293 Cas9 cells (panels b,e) and HEK-293 NEFM KO cells (panel c,f). Cells were fixed, permeabilized, and labelled with NEFM Recombinant Rabbit Monoclonal Antibody (JM11-20) (Product # MA5-32613) (1:500 dilution), followed by Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) (1:2,000). Nuclei (blue) were stained using ProLong™ Diamond Anti... View More
Product Details
MA5-32613
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JM11-20
Immunogen
Synthetic peptide within Human NEFM aa 651-692
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809890
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 160 kDa neurofilament protein; Neurofilament 3; neurofilament 3, medium; Neurofilament medium polypeptide; neurofilament protein M; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); neurofilament-M; NF-M; NMC
Gene Aliases: NEF3; NEFM; NF-M; NF160; NF165; NFM
UniProt ID: (Human) P07197, (Mouse) P08553, (Rat) P12839
Entrez Gene ID: (Human) 4741, (Mouse) 18040, (Rat) 24588
neurofilament bundle assembly
axon development
mitophagy in response to mitochondrial depolarization
microtubule cytoskeleton organization
axo-dendritic transport
regulation of axon diameter
intermediate filament cytoskeleton organization
intermediate filament polymerization or depolymerization
intermediate filament bundle assembly
neurofilament cytoskeleton organization
spinal cord development
axon regeneration
cellular response to oxidative stress
cellular response to estradiol stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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