Invitrogen

NeuroD1 Recombinant Rabbit Monoclonal Antibody (JM11-10)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (28) NeuroD1 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-32626

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Immunoprecipitation (IP)

1:10-1:50
-

ChIP assay (ChIP)

2.5 µg/10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JM11-10

Immunogen

Synthetic peptide within Human NeuroD1 aa 1-44
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809903

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

NeuroD1 is a transcriptional activator that acts as a differentiation factor during neurogenesis. It has been demonstrated to bind to the insulin gene E-box. Efficient DNA binding requires dimerization with another basic helix-loop-helix (bHLH) protein. Defects in NEUROD1 are a cause of maturity onset diabetes of the young type VI (MODY6). MODY6 is a form of non-insulin-dependent diabetes mellitus characterized by an autosomal dominant mode of inheritance, onset during young adulthood and a primary defect in insulin secretion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: Basic helix-loop-helix factor 1; basic helix-loop-helix transcription factor; beta-cell E-box transactivator 2; Beta-cell E-box transcriptional activator 2; Beta2; BHF-1; BHF1; bHLHa3; Class A basic helix-loop-helix protein 3; neuro d1; NeuroD; NeuroD1; neurogenic differentiation 1; Neurogenic differentiation factor 1; neurogenic helix-loop-helix protein NEUROD

View more View less

Gene Aliases: BETA2; BHF-1; BHLHA3; MODY6; NEUROD; NEUROD1

View more View less

UniProt ID: (Human) Q13562, (Rat) Q64289, (Mouse) Q60867

View more View less

Entrez Gene ID: (Human) 4760, (Rat) 29458, (Mouse) 18012

View more View less

Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding transcription factor binding sequence-specific DNA binding protein heterodimerization activity E-box binding DNA binding double-stranded DNA binding protein dimerization activity basic helix-loop-helix transcription factor
Process(es)
pancreatic A cell fate commitment pancreatic PP cell fate commitment transcription from RNA polymerase II promoter nucleocytoplasmic transport nitric oxide mediated signal transduction response to glucose anterior/posterior pattern specification dentate gyrus development cerebellum development neurogenesis signal transduction involved in regulation of gene expression insulin secretion endocrine pancreas development amacrine cell differentiation enteroendocrine cell differentiation response to drug glucose homeostasis positive regulation of apoptotic process positive regulation of cell differentiation positive regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of JAK-STAT cascade embryonic organ morphogenesis neuron development inner ear development regulation of insulin secretion positive regulation of sequence-specific DNA binding transcription factor activity regulation of intestinal epithelial structure maintenance regulation of cell cycle arrest cellular response to glucose stimulus negative regulation of type B pancreatic cell apoptotic process positive regulation of transcription regulatory region DNA binding transcription, DNA-templated hindbrain development camera-type eye development cell fate commitment regulation of neuron differentiation regulation of transcription, DNA-templated multicellular organism development nervous system development cell differentiation
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support