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Invitrogen
IGF1R (CD221) Recombinant Rabbit Monoclonal Antibody (012)
FIGURE: 1 / 2
IGF1R (CD221) Antibody (MA5-43111) in ICC/IF
Immunocytochemistry analysis of IGF1R (CD221) in MCF7 cells. Samples were treated with 4% PFA, permeabilized with 1% Triton X-100 in PBS, and blocked with 10% serum, incubated in IGF1R (CD221) monoclonal antibody (Product # MA5-43111) at a dilution of 1:60, followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG.
Product Details
MA5-43111
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
012
Immunogen
Recombinant Human IGF1R/CD221 protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2912248
Product Specific Information
Specificity: Human IGF1R/CD221
Target Information
IGF1R, also known as Insulin-like Growth Factor 1 (IGF-1) Receptor, is a receptor tyrosine kinase activated by IGF-1 and IGF-2. IGFR has been implicated in a variety of cancers. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD221; CD221 antigen; IGF 1 receptor; IGF 1R; IGF-I receptor; Insulin like growth factor 1 receptor precursor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2
Gene Aliases: CD221; IGF1R; IGFIR; IGFR; JTK13
UniProt ID: (Human) P08069
Entrez Gene ID: (Human) 3480
protein tyrosine kinase activity
insulin-like growth factor-activated receptor activity
insulin receptor binding
protein binding
insulin-like growth factor binding
ATP binding
insulin-like growth factor I binding
identical protein binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
transmembrane signal receptor
immune response
signal transduction
positive regulation of cell proliferation
insulin receptor signaling pathway
phosphatidylinositol 3-kinase signaling
positive regulation of cell migration
peptidyl-tyrosine autophosphorylation
negative regulation of apoptotic process
positive regulation of DNA replication
regulation of JNK cascade
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
protein tetramerization
inactivation of MAPKK activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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