Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 4
HSP60 Antibody (MA5-45110) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using HaCaT cells. Fixation involved Cold 100% methanol at -20°C for 10 minutes. Samples were incubated with Hsp60 monoclonal antibody (Product # MA5-45110) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.
Product Details
MA5-45110
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Fruit fly,
Guinea pig,
Hamster,
Human,
Insect,
Mouse,
Non-human primate,
Sheep,
Plant,
Pig,
Rabbit,
Rat,
Shrew,
Xenopus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
LK1
Immunogen
Recombinant human HSP60
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.09mM sodium bicarbonate/PBS with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931564
Product Specific Information
0.05 µg/mL of MA5-45110 was sufficient for detection of HSP60 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody. |Detects approximately 60kDa.
Target Information
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; 60-kDa heat shock protein, mitochondrial; CG12101-PA; CG12101-PB; chaperonin; Chaperonin 60; CPN60; heat shock 60 kDa protein 1; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); Heat shock protein; heat shock protein 1 (chaperonin); Heat shock protein 60; heat shock protein 60 (liver); heat shock protein 60 kDa; Heat shock protein 60A; heat shock protein 65; Heat shock protein family D member 1; heat shock protein, 60 kDa; HSP; Hsp 60; HSP-60; HSP-65; hsp60; Hsp60A-PA; Hsp60A-PB; Hsp60s1; HuCHA60; lethal(1)10Ac; mitochondrial heat shock 60 kDa protein 1; Mitochondrial matrix protein P1; P1 protein; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1
Gene Aliases: 12; 60kDa; BP5; CG12101; chaperonin; CPN60; d-hsp60; Dm10A; Dmel\CG12101; Dmel_CG12101; DmHsp60; G62; GROEL; HLD4; Hsp 60; HSP-60; HSP60; HSP60A; Hsp64; HSP65; HSPD1; Hspd1-30p; HuCHA60; I79_011398; IEF16; l(1)10Ac; l(1)BP5; l(1)dp025; l(1)G8; l(1)HM21; l(1)L12; Mmp-P1; RCJMB04_7g5; SPG13; SSP 7506; XELAEV_18045048mg
ATP binding
isomerase activity
lipopolysaccharide binding
p53 binding
double-stranded RNA binding
protein binding
high-density lipoprotein particle binding
ubiquitin protein ligase binding
apolipoprotein A-I binding
chaperone binding
DNA replication origin binding
single-stranded DNA binding
ATPase activity
poly(A) RNA binding
unfolded protein binding
nucleotide binding
protease binding
protein complex binding
insulin binding
protein heterodimerization activity
misfolded protein binding
B cell cytokine production
MyD88-dependent toll-like receptor signaling pathway
positive regulation of T cell mediated immune response to tumor cell
protein folding
activation of cysteine-type endopeptidase activity involved in apoptotic process
apoptotic mitochondrial changes
response to cold
positive regulation of interferon-alpha production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
mitochondrial unfolded protein response
protein refolding
B cell proliferation
T cell activation
positive regulation of macrophage activation
negative regulation of apoptotic process
protein import into mitochondrial intermembrane space
isotype switching to IgG isotypes
protein stabilization
positive regulation of T cell activation
interaction with symbiont
cellular response to interleukin-7
detection of misfolded protein
response to unfolded protein
B cell activation
positive regulation of apoptotic process
negative regulation of neuron apoptotic process
positive regulation of inflammatory response
chaperone mediated protein folding requiring cofactor
'de novo' protein folding
viral process
chaperone-mediated protein complex assembly
protein maturation
protein targeting to mitochondrion
mitochondrion organization
response to heat
cellular response to heat
response to hypoxia
response to organic substance
response to organic cyclic compound
response to lipopolysaccharide
response to ATP
response to cocaine
response to drug
response to hydrogen peroxide
response to estrogen
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support