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DNMT1 Antibody (MA5-45293) in IHC
Immunohistochemistry analysis using medullar kidney tissue. Samples were incubated with DNMT1 monoclonal antibody (Product # MA5-45293) at 1:1,000, followed by HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown). Counterstain used was Mayer Hematoxylin (purple/blue) nuclear stain.
Product Details
MA5-45293
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fish,
Human,
Mouse,
Zebrafish
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
60B1220.1
Immunogen
Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
47.35mM phosphate/2.15mM MES with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931747
Product Specific Information
2 µg/mL of MA5-45293 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 180kDa. It will cross-react with mouse DNMT1.
Target Information
This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CXXC-type zinc finger protein 9; DNA (cytosine-5)-methyltransferase 1; DNA methyltransferase HsaI; DNA methyltransferase MmuI; DNA MTase HsaI; DNA MTase MmuI; Dnmt1; FLJ16293; MCMT; MGC104992
Gene Aliases: ADCADN; AIM; cb1075; cb983; CXXC9; ddn; dmnt1; DNMT; DNMT1; Dnmt1o; fb05h01; HSN1E; m.HsaI; m.MmuI; MCMT; Met-1; Met1; MommeD2; mta; MTase; Uim; wu:fb05h01
UniProt ID: (Human) P26358, (Mouse) P13864
Entrez Gene ID: (Human) 1786, (Mouse) 13433, (Zebrafish) 30430
DNA binding
double-stranded DNA binding
RNA binding
DNA (cytosine-5-)-methyltransferase activity
protein binding
zinc ion binding
methyl-CpG binding
DNA-methyltransferase activity
protein domain specific binding
estrogen receptor binding
histone deacetylase binding
unmethylated CpG binding
DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates
promoter-specific chromatin binding
chromatin binding
catalytic activity
transcription factor binding
methyltransferase activity
transferase activity
metal ion binding
DNA metabolism protein
DNA methyltransferase
nucleic acid metabolism protein
negative regulation of transcription from RNA polymerase II promoter
DNA methylation
transcription, DNA-templated
Ras protein signal transduction
brain development
aging
response to heat
response to toxic substance
response to ionizing radiation
maintenance of DNA methylation
DNA methylation on cytosine within a CG sequence
positive regulation of gene expression
response to activity
gene silencing
covalent chromatin modification
neuron differentiation
response to caffeine
response to estradiol
response to lipopolysaccharide
response to vitamin A
response to testosterone
cellular response to platelet-derived growth factor stimulus
response to antidepressant
regulation of cell proliferation
DNA hypermethylation
response to ethanol
negative regulation of gene expression, epigenetic
S-adenosylhomocysteine metabolic process
S-adenosylmethioninamine metabolic process
S-adenosylmethionine metabolic process
positive regulation of histone H3-K4 methylation
negative regulation of histone H3-K9 methylation
cellular response to amino acid stimulus
cellular response to lead ion
cellular response to transforming growth factor beta stimulus
C-5 methylation of cytosine
positive regulation of methylation-dependent chromatin silencing
cellular response to nerve growth factor stimulus
regulation of transcription, DNA-templated
metabolic process
regulation of gene expression
chromatin modification
methylation
DNA methylation on cytosine
negative regulation of transcription, DNA-templated
liver development
lens development in camera-type eye
determination of left/right symmetry
retina layer formation
pancreas development
exocrine pancreas development
intrahepatic bile duct development
regulation of DNA methylation
digestive tract development
cartilage development
histone H3-K9 methylation
retina morphogenesis in camera-type eye
definitive hemopoiesis
Kupffer's vesicle development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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