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VGLUT2 Antibody (MA5-45510) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with VGLUT2 monoclonal antibody (Product # MA5-45510) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Syn... View More
Product Details
MA5-45510
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N29/29
Immunogen
Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931964
Product Specific Information
1 µg/mL of MA5-45510 was sufficient for detection of VGLut2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 60kDa.
This antibody was formerly sold as clone S29-29.
Target Information
VGLU2 mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. This protein may also mediate the transport of inorganic phosphate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: differentation-associated Na-dependent inorganic phosphate cotransporter; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; differentiation-associated Na-dependent inorganic phosphate cotransporter; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6; solute carrier family 17 (vesicular glutamate transporter), member 6; Solute carrier family 17 member 6; Vesicular glutamate transporter 2; VGlU2; VGluT2
Gene Aliases: 2900073D12Rik; DNPI; SLC17A6; VGLUT2
UniProt ID: (Human) Q9P2U8, (Mouse) Q8BLE7, (Rat) Q9JI12
Entrez Gene ID: (Human) 57084, (Mouse) 140919, (Rat) 84487
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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