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Invitrogen
4-Hydroxynonenal Monoclonal Antibody (12F7), PE
FIGURE: 1 / 1
4-Hydroxynonenal Antibody (MA5-45792) in WB
Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal protein. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: 4-hydroxyl nonenal-BSA (0.5 µg). Lane 4: 4-hydroxy nonenal-BSA (2.0 µg). Lane 5: 4-hydroxy-2-hexenal (0.5 µg). Lane 6: 4-hydroxy-2-hexenal (2.0 µg). Blocking: 5% Skim Milk in TBST. Samples were incubated with 4-hydroxy-nonenal monoclonal antibody (Product # MA5-45792) at 1:1,000 for 2 hours at RT, followed by Goat Anti-Mouse IgG: HRP at 1:2,000 for 60 min at RT. ... View More
Product Details
MA5-45792
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
12F7
Immunogen
Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
47.35mM phosphate/2.15mM MES, pH 7.4, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932246
Product Specific Information
A 1:1,000 dilution of MA5-45792 was sufficient for detection of 4-Hydroxynonenal in 0.5 µg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
4-Hydroxynonenal, or 4-hydroxy-2-nonenal or 4-HNE or HNE, is an alpha, beta-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary alpha, beta-unsaturated hydroxyalkenal formed in this process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 4-HNE; 4-hydroxy Hexenal; 4-Hydroxy Nonenal; 4-hydroxy-2-nonenal; 4-Hydroxynonenal; 4HNE; HNE
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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