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Invitrogen
7-Ketocholesterol Monoclonal Antibody (3F7), FITC
FIGURE: 1 / 1
7-Ketocholesterol Antibody (MA5-45798) in WB
Western Blot analysis of 7-Ketocholesterol-BSA Conjugate showing detection of 67 kDa 7-Ketocholesterol protein. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (2.0 µg). Lane 4: 7-ketocholesterol-BSA (0.5 µg). Lane 5: 7-ketocholesterol-BSA (2.0 µg). Blocking: 5% Skim Milk in TBST. Samples were incubated with 7-Ketocholesterol monoclonal antibody (Product # MA5-45798) at 1:1,000 for 2 hours at RT, followed by Goat Anti-Mouse IgG: HRP at 1:2,000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predic... View More
Product Details
MA5-45798
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
3F7
Immunogen
Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932252
Product Specific Information
A 1:1,000 dilution of MA5-45798 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for 7-ketocholesterol modified proteins.
Target Information
7-Ketocholesterol is a major oxidation product of cholesterol found in human atherosclerotic plaque and is more atherogenic than cholesterol in some animal studies. 7-Ketocholesterol can inhibit cholesterol 7alpha-hydroxylase, the rate-limiting step in bile acid biosynthesis, as well as strongly inhibiting HMG-CoA reductase, the rate-limiting enzyme in cholesterol biosynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 7-KC; 7KC; 7KC-Modified KLH
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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