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Metallothionein Antibody (MA5-45899) in WB
Western Blot analysis of Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged showing detection of 36 kDa Metallothionein protein. Lane 1: Molecular Weight Ladder (MW). Lane 2: GST. Lane 3: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged. Load: 1 µg. Blocking: 5% Skim Milk powder in TBST. Samples were incubated with Metallothionein monoclonal antibody (Product # MA5-45899) at 1:1,000 for 2 hours at RT, followed by Goat Anti-Mouse IgG:HRP at 1:5,000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Pre... View More
Product Details
MA5-45899
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2B5
Immunogen
Recombinant full length Pseudomonas aeruginosa Metallothionein protein
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932353
Product Specific Information
A 1:1,000 dilution of MA5-45899 was sufficient for detection of Metallothionein in 1 µg of purified Pseudomonas aeruginosa Metallothionein (PmtA)-GST tagged protein by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects 36 kDa band corresponding to Pseudomonas aeruginosa Metallothionein.
Target Information
Metallothioneins (MTs) are a family of low molecular weight, heavy metal-binding proteins characterized by a high cysteine content and lack of aromatic amino acids. MTs bind 7 to 12 heavy metal atoms per molecule of protein. They are ubiquitous in the animal and plant kingdoms and are also found in prokaryotes. In mammals, the cysteine residues are absolutely conserved and serve to coordinate heavy metal atoms such as zinc, cadmium, and copper via mercaptide linkages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CES 1; CES1; Metallothionein 2; Metallothionein 2A; Metallothionein IA; Metallothionein II; Metallothionein2; MGC32848; MT 1A; MT 2; MT 2A; MT IA; MT II; MT2; MT2A; Probable pmtA
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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