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MHC Class II Antibody (MA5-46188) in Flow
Flow Cytometry analysis of MHC Class II using MHC Class II Monoclonal Antibody (M5/114), Biotin (Product # MA5-46188). Separation of murine MHC Class II positive splenocytes (red-filled) from MHC Class II negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-mouse MHC Class II (M5/114) Biotin antibody (concentration in sample 9 μg/mL, Streptavidin APC).
Product Details
MA5-46188
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
M5/114
Immunogen
Activated C57BL/6 mouse spleen cells
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2937581
Product Specific Information
This antibody recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Target Information
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MHC 2; MHC Class II; MHC II; MHCCII
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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